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文章 食管癌相关文章ESCC

..., Liao Q, Zhao Y, Yuan Y. Comprehensive landscape of resistance mechanisms for neoadjuvant therapy in esophageal squamous cell carcinoma by single-cell transcriptomics. Signal Transduct Target Ther. 2023 Aug 11;8(1):298. [4] Jia Y, Zhang B, Zhang C, Kwong DL, Chang Z, Li S, Wang Z, Han H, Li J, Zh...

文章 转录组测序分析水稻旱作的重要基因

...Babu & Polumetla A. Kumar RNA-seq reveals the involvement of key genes for aerobic adaptation in rice. Scientific Reports volume 9, Article number: 5235 (2019) 推荐： 二代测序转录组数据自主分，转录组标准分析后的数据挖掘、转录组文献解读 更多生物信...

文章 一文教你绘制九象限图

...devtools") # 判断R包加载是否成功来决定是否安装后再加载 for(p in package_list){ if(!suppressWarnings(suppressMessages(require(p, character.only = TRUE, quietly = TRUE, warn.conflicts = FALSE)))){ install.packages(p, warn.conflicts = FALSE) suppressWarnings(suppressMessa...

文章 envfit()的线性拟合：非约束排序（PCA、CA、PCoA、NMDS）

...mary(tbpca, scaling = 1)$sites[ ,1:2])tbpca_env_sign <- tbpca_env[which(tbpca_env$p.adj < 0.05),1:2] #PCA 前两轴贡献度（%）tbpca_eig <- round(100 * tbpca$CA$eig[1:2] / sum(tbpca$CA$eig), 2) #读取样本分组数据（附件“group.txt”，第一列为各样本...

文章 8.28接收-毛竹bHLH基因家族分析文章解读及原文献下载

...： a: The 137 PebHLH genes are clustered into 16 subfamilies. b: Comparison of bHLH family members from Moso bamboo, rice and Arabidopsis. Different colors represent the different species, and the percentage of each subfamily in each species is shown. Black: AtbHLH genes; Red: OsbHLH genes; Blu...

文章 基因编辑iPSCS敲除技术原理与潜在的治疗应用

...L A, et al. Using CRISPR-Cas9 to Generate Gene-Corrected Autologous iPSCs for the Treatment of Inherited Retinal Degeneration[J]. Molecular Therapy, 2017, 25(9): 1999-2013. Xu H, Wang B, Ono M, et al. Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compati...

文章 转录组扫盲系列--reads如何比对到参考基因组上？

...基因组的区域以便于去寻找那些含有剪接信号(GT-AG, GC-AG, or AT-AC))的剪接位点；（b）串联间隔序列：将步骤（a）中找到的潜在的剪接位点的侧翼基因组序列被串联起来，并构建索引，未映射的read片段用Bowtie2比再次比对串联的侧...

文章 Nature Medicine同期8篇论文，聚焦人工智能在医学领域的应用

...领域的应用。BioWorld列举并介绍了这8篇论文： 1 High-performance medicine: the convergence of human and artificial intelligence 高效医学：人类与智能的融合 作者：Eric J. Topol；单位：Scripps研究所 通过在所有部门中使用标记的大数据以及显...

文章 biom格式的文件如何转化

...on-v2.7.11/bin/biom", line 9, in <module> load_entry_point('biom-format==2.1.5', 'console_scripts', 'biom')() File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/site-packages/click-6.3-py2.7.egg/click/core.py", line 716, in __call__ return self.main(*args, **kwargs) File...

文章 绘制全基因组甲基化图谱

...",required=F,default=7,                help="the font size of text for axis [optional, default:7]",                metavar="size")parser$add_argument("-T", "--title", type="character",required=F, default="",                help="the main title",                metavar...

文章 R脚本绘制多层环形饼图

... brewer.pal(5, "Set2") names(color_palette) <- unique(sb$rects$browser[!is.na(sb$rects$browser)]) sb$rects$color <- color_palette[sb$rects$browser] #利用sunburst函数绘图 p <- sunburst(sb, rects.fill=sb$rects$color, node_labels = T, node_labels.min = 15) p + geom_text(data = sb$leaf_la...

文章 两个诺贝尔奖强强联合--CRISPR基因编辑的ESC/iPSC应用于再生和个性化医学

...步“！ 参考文献: CRISPR/Cas9-based targeted genome editing for correction of recessive dystrophic epidermolysis bullosa using iPS cells. PNAS, vol. 116(52), 26846–26852, December 26, 2019. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Ki...

文章 GATK外显子测序数据肿瘤体细胞突变数据分析

...humandb/hg38/可到ANNOVAR官方网站下载： https://annovar.openbioinformatics.org/en/latest/user-guide/download/ 命令行如下： table_annovar.pl 154.clean.vcf.gz  humandb/hg38/ \ -buildver hg38 -out 154 -remove -protocol refGene,cosmic70,nci60,esp6500siv2_all,clinvar_2021050...

文章 排序分析PCA、PCoA、CA、NMDS、RDA、CCA等区别与联系

...序排序区别 非约束排序也叫间接梯度分析（unconstrained ordination or indirect gradient analysis）的目标就是发现这样的坐标轴，让群落中的样方或是物种的最大变化量能够在坐标轴上体现出来。换句话说，让尽可能多的变化量能够在尽...

文章 必须收藏！比较基因组学分析必备神器-OrthoVenn2

...g Q Gu, Devin Coleman-Derr, Qingyou Xia, Yi Wang. OrthoVenn2: a web server for whole-genome comparison and annotation oforthologous clusters across multiple species, Nucleic Acids Research, Volume 47, Issue W1, 02 July 2019, Pages W52–W58, https://doi.org/10.1093/nar/gkz333. 日常科研中你我...