a, UMAP (uniform manifold approximation and projection) of germ cell in the human (top; n = 10,993), mouse (middle; n = 10,411) and external macaque (bottom: n = 2,685) scRNA-seq datasets labelled by germ cell states, sex, donor/sample identity and developmental stage indicated in post-conceptional weeks (PCW) or embryonic (E) / postnatal (P) days). Doublets and low QC cells removed. b, Downsampled UMAP for human germ cells to account for up to 50 cells per donor (colour) for confirmatory visualisation. c, Dot plots showing the variance-scaled, log-transformed expression of genes characteristic of fetal oogenesis in the human (top), mouse (middle) and macaque (bottom) germ cells scRNA-seq data. d, Relative proportion of human germ cell states (colour) profiled with scRNA-seq, classified by sex and developmental stage. e, t-SNE (t-distributed stochastic neighbour embedding) projection of scRNA-seq data of human germ cells coloured by Palantir pseudo-time and probability of cells to progress from the PGC status. Germ cells are downsampled to account for 500 cells for each germ cell state. E = embryonic day; FGC = fetal germ cells; P = postnatal day; PCW = post-conceptional weeks; PGC = primordial germ cells.
a，人类（上方;n = 10,993）、小鼠（中位;n = 10,411）和外部猕猴（下：n = 2,685）中生殖细胞的 UMAP（统一流形近似与投影），这些数据集按生殖细胞状态、性别、供体/样本身份及发育阶段标注于受孕后周（PCW）或胚胎（E）/出生后（P）日）。去除双重体和低质量细胞。b、对人类生殖细胞进行下采样 UMAP，以考虑每个供体最多 50 个细胞（颜色），用于确认性可视化。c，点图显示了人类（上方）、小鼠（中间）和猕猴（下方）生殖细胞 scRNA-seq 数据中胎儿卵子发生特征基因的方差尺度对数转化表达。d、用 scRNA-seq 分析的人类生殖细胞状态（颜色）的相对比例，按性别和发育阶段分类。e， t-SNE（t-分布随机邻近嵌入）对人类生殖细胞 scRNA-seq 数据的投影，这些数据通过 Palantir 伪时间和细胞从 PGC 状态进展的概率着色。生殖细胞被下采样，以对应每个生殖细胞状态的 500 个细胞。E = 胚胎日;FGC = 胎儿生殖细胞;P = 产后日;PCW = 受孕后周;PGC = 原始生殖细胞。

参考文献：https://www.nature.com/articles/s41586-022-04918-4/figures/9