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文章 转录组个性化分析之可变剪切分析工具:rMATS turbo

...  --task {prep,post,both,inte}                        Specify which step(s) of rMATS to run. Default: both.                        prep: preprocess BAMs and generate a .rmats file.                        post: load .rmats file(s) into memory, detect and      ...

文章 R 包安装报错:configure: error: gdal-config not found or not executable.

...k/biosoft/gcc/gcc-v6.4.0/bin/gcc configure: CXX: g++ -std=gnu++11 checking for gdal-config... no no configure: error: gdal-config not found or not executable. ERROR: configuration failed for package ‘sf’ * removing ‘/share/work/biosoft/R/R-v3.6.1/lib64/R/library/sf’ ERROR: dependency ‘sf...

问题 TCGA的RNA-seq count annotation过滤,有些不知道是否需要删除

...ontamination, or low alignment rate) are well below average for this file, which should be used with caution, if at all, for expression analysis. However, other analyses, e.g. mutation and structural variant calling, may still give reliable result. 4. aliquot_annotation_updated:Annotation refers...

文章 NCBI的SRA Lite和SRA Normalized数据有什么区别

...A文件的另一个格式(https://www.ncbi.nlm.nih.gov/sra/docs/sra-data-formats/) 我们平常下载的SRA文件属于SRA Normalized Format,它和SRA Lite之间最大的区别就是SRA Lite采用了简化的quality scores,具体它是如何简化的请参考原文 SRA Lite files are produ...

文章 windows10中基于WSL2的docker镜像存储位置更改

...ure it to utilize drive D: instead. There is a Microsoft documentation, which describes a WSL configuration file, but it seems to be a bit old these days. At the same time I couldn’t find anything useful on Docker website itself. What I finally spot was this tricky solution from Franks Chow ...

问题 非模式物种建立注释库问题

报错信息:ℹ Use `spec()` to retrieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(...

文章 Evolview绘制进化树时添加热图

...数说明: The following 'modifiers' (Key-Value pairs) are supported for heatmap:universal modifiers!Title or !Legendtexttitle of the legend; default = name of the dataset!ShowLegends0 or 10 : hide legends; 1 : show legends!opacityfloat number between 0 to 1opacity of the datasetmodifiers share...

文章 iTOL进化树外层添加圆环

...g with a hash are comments and ignored during parsing#select the separator which is used to delimit the data below (TAB,SPACE or COMMA).This separator must be used throught this file (except in the SEPARATOR line, which uses space).#SEPARATOR TABSEPARATOR SPACE#SEPARATOR COMMA#label is used in the l...

文章 blastall 参数详细说明

...开gap得分[Integer]-1 -E延伸gap得分[Integer]-1 -XX dropoff value for gapped alignment (in bits)[Integer]0blastn 30, megablast 20, tblastx 0, all others 15-I显示gi号Show GI’s in deflines[T/F]F -q核酸错配罚分[Integer]-3blastn only-r核酸匹配得分[Integer]1blastn only-vNumber of...

文章 HMMER ——hmmsearch 用法,参数

...asenhmmscan: search DNA sequence against a DNA profile databasehmmfetch: retrieve profile(s) from a profile filehmmstat: show summary statistics for a profile filehmmemit: generate (sample) sequences from a profilehmmlogo: produce a conservation logo graphic from a profilehmmconvert: convert b...

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问题 老师好,我在做群里遗传进化算pca时,得到的文件都是0。但是没有报错

...iants loaded from .bim file.2045265 missing IDs set.684 people (0 males, 0 females, 684 ambiguous) loaded from .fam.Ambiguous sex IDs written to plink_pca.nosex .Using up to 23 threads (change this with --threads).Before main variant filters, 684 founders and 0 nonfounders present.Calculating allele...

问题 使用NCBI下载的基因组和gff文件进行基因家族分析时,保留编码蛋白基因的命令似乎无法识别NCBI的gff文件(描述中为日志,图片为尝试获取的gff文件)

...the child features to reflect this change. Why? because we do not know to which L1 the child feature was part-of because several Parent have similar ID.  @ the feature is: NC_049979.1     RefSeq  cDNA_match      22755   22861   .       -       .       ID "nbis-cdna_match-1...

文章 MA plot 绘图计算公式

...ies and see whether the slope of the line is around 1. An improved method, which is basically a scaled, 45 degree rotation of the R vs. G plot is an MA-plot.[4] The MA-plot is a plot of the distribution of the red/green intensity ratio ('M') plotted by the average intensity ('A'). M and A are defin...