找到约 15 条结果

问题 TCGA的RNA-seq count annotation过滤,有些不知道是否需要删除

.... patient_annotation:Notification:Item in special subset:This is part of the ovarian triplet set. 3. aliquot_annotation:CenterNotification:Item flagged DNU:QC Warning- Expression quality metrics (e.g. 3-prime bias, high duplicate rate, genomic contamination, or low alignment rate) are well bel...

问题 如何用荧光定量数据绘制热图?

...n profiles of PtSAUR genes in different tissues as determined by qRT-PCR. There were three biological and three technicalreplicates performed for each sample. The relative expression levelswere evaluated by 2-ΔΔCT. GraphPad software was used for the statistical analyses. 作者是用的2-ΔΔCT...

文章 perl中判断数组中是否含有某个元素

...or; if (grep { $visitor eq $_ } @names) { print "Visitor $visitor is in the guest list\n"; } else { print "Visitor $visitor is NOT in the guest list\n"; } 在这个例子中,grep函数位于标量上下文。在标量上下文中,grep返回通过过滤的元素个数。我们检查的条...

文章 生信分析-小提琴图绘制-脚本使用

...p show this help message and exit -m meta, --meta meta Input the clinical information file path that contains the grouping[required] -n name, --name name The column name of the value[optional,default:IRPS] -g group_name, --group_name group_name ...

问题 运行GDCprepare(query, save=FALSE)时错误

....category = "Transcriptome Profiling", +                   data.type = "Gene Expression Quantification",  +                   workflow.type = "STAR - Counts") > queryFt <- GDCquery(project = "TCGA-BRCA", +                   data.category = "Transcriptome Prof...

文章 Minimap2安装与使用

...ase quality)reads碱基质量值12 Optional Fields 可选的列以TAG:TYPE:VALUE的形式提供额外的信息 (三)FLAG释义和CIGAR string 1、FLAG释义,常见于bam文件中 1 : 代表这个序列采用的是PE双端测序2: 代表这个序列和参考序列完全匹配,...

文章 BIOM 微生物数据格式及文件转换

...JSON格式 biom convert -i table.txt -o table.from_txt_json.biom --table-type="OTU table" --to-json biom convert -i table.txt -o table.from_txt_hdf5.biom --table-type="OTU table" --to-hdf5 转换biom为经典格式 biom convert -i table.biom -o table.from_biom.txt --to-tsv 转换biom为经典格...

问题 你好,打扰了,想请教一下我是mac版R studio,安装WGCNA总报错,不太看得懂哪里出了问题,麻烦指导一下,急,谢谢!

...开URL’https://cran.rstudio.com/src/contrib/WGCNA_1.69.tar.gz' Content type 'application/x-gzip' length 1168289 bytes (1.1 MB) ================================================== downloaded 1.1 MB * installing *source* package ‘WGCNA’ ... ** 成功将‘WGCNA’程序包解包并MD5和...

文章 seurat_sc_cluster.r 脚本使用帮助:

...d count file , csv or tsv format[ optional]  --sep sep             the field separator character for count file; Values                        on each line of thefile are separated by this                        character. If ‘sep = ""’ (the default for       ...

文章 地球上物种总数到底有多少??

...献及来源:Mora C, Tittensor D P, Adl S, et al. How Many Species Are There on Earth and in the Ocean?[J]. Plos Biology, 2011, 9(8):e1001127.Locey K J, Lennon J T. Scaling laws predict global microbial diversity[J]. Proceedings of the National Academy of Sciences of the United States of America, ...

问题 GWAS中gapit分析没有结果,命令行输入与屏幕输出如下

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Loading required package: BiocGenerics Attaching package: ‘BiocGenerics’ The...

问题 老师,您好!key driver analysis的参考文献如下。请您帮我查查该分析方法的R语言包在哪里下载?

Identification of Key Causal Regulators in Gene Networks . Bin Zhang and Jun Zhu .Proceedings of the World Congress on Engineering 2013 Vol II, .

文章 安装picrust2 保存:pkg_resources.DistributionNotFound: The 'cython' distribution was not found and is required by biom-format

...: All installation instructions are in more detail here. First, clone the repository and enter the directory: git clone https://github.com/picrust/picrust2.git cd Next, install the required dependencies listed here. Install PICRUSt2 into it's own environment using conda: conda env create...

文章 快速高效KM生存曲线绘制-脚本使用

... vs 女等)对患者生存造成的影响。 例如:2021年发表在The Journal for ImmunoTherapy of Cancer上的一篇影响因子为13.75的文献为例,该文献中运用KM生存曲线生动形象地展现了不同变量分别对患者生存的影响。 作者根据基因表达量和细...

问题 重测序circos运行失败

...rence/best_practices   The debugging facility is helpful to figure out what's happening under the   hood       http://www.circos.ca/documentation/tutorials/configuration/debugging   If you're still stumped, get support in the Circos Google Group.       http://groups.google.com/...