如何用荧光定量数据绘制热图?

attachments-2019-10-XOJBDmd65db6b489073f2.png这是看到的一篇文献中Fig. 6. Expression profiles of PtSAUR genes in different tissues as determined by qRT-PCR.

There were three biological and three technical
replicates performed for each sample. The relative expression levels
were evaluated by 2
-ΔΔCT. GraphPad software was used for the statistical analyses.

作者是用的2-ΔΔCT的方法做的数据分析,可是结果怎么会是负数呢?

在问答社区内看到attachments-2019-10-S3WguvDN5db6b574e80bc.png莫非他是用的-ΔΔCT?恳请老师们解答,谢谢了

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最佳答案 2019-10-29 15:36

为了是热图每一行对比明细,使热图很好看,热图一般按行均一化数据。所以,legend会有负数。聚类热图绘制见:https://www.omicsclass.com/article/528

qpcr的计算方法见:https://www.omicsclass.com/article/446

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  • 。翟 提出于 2019-10-28 17:33

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