clustalw2多序列比对,序列输入不进去


在linux系统下,我输入clustalw2命令, 返回以下提示:
**************************************************************
******** CLUSTAL 2.1 Multiple Sequence Alignments  ********
**************************************************************


     1. Sequence Input From Disc
     2. Multiple Alignments
     3. Profile / Structure Alignments
     4. Phylogenetic trees

     S. Execute a system command
     H. HELP
     X. EXIT (leave program)
Your choice:

我输入1, 回车,出现:
Sequences should all be in 1 file.

7 formats accepted:
NBRF/PIR, EMBL/SwissProt, Pearson (Fasta), GDE, Clustal, GCG/MSF,                  RSF.


Enter the name of the sequence file :

我输入了我的fasta格式的序列文件名,回车,出现:


Sequence format is Pearson



**************************************************************
******** CLUSTAL 2.1 Multiple Sequence Alignments  ********
**************************************************************


     1. Sequence Input From Disc
     2. Multiple Alignments
     3. Profile / Structure Alignments
     4. Phylogenetic trees

     S. Execute a system command
     H. HELP
     X. EXIT (leave program)


Your choice:
我选择2,回车,出现:
****** MULTIPLE ALIGNMENT MENU ******


    1.  Do complete multiple alignment now Slow/Accurate
    2.  Produce guide tree file only
    3.  Do alignment using old guide tree file

    4.  Toggle Slow/Fast pairwise alignments = SLOW

    5.  Pairwise alignment parameters
    6.  Multiple alignment parameters

    7.  Reset gaps before alignment? = OFF
    8.  Toggle screen display          = ON
    9.  Output format options
    I. Iteration = NONE

    S.  Execute a system command
    H.  HELP
    or press [RETURN] to go back to main menu


Your choice:
我输入1,回车,结果1被删除,程序不运行,试了无数次都是这样,哪位知道这是为什么呀?谢谢了!
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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

检查你输入的序列里面的的ID是否有重复的,如果有重复的会出现输入不进去。

参考:https://www.omicsclass.com/question/1621

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  • 提出于 2020-01-09 14:35