clustalw2多序列比对，序列输入不进去

在linux系统下,我输入clustalw2命令, 返回以下提示:
**************************************************************
******** CLUSTAL 2.1 Multiple Sequence Alignments  ********
**************************************************************

   1. Sequence Input From Disc
   2. Multiple Alignments
   3. Profile / Structure Alignments
   4. Phylogenetic trees

   S. Execute a system command
   H. HELP
   X. EXIT (leave program)
Your choice:

我输入1, 回车,出现:
Sequences should all be in 1 file.

7 formats accepted:
NBRF/PIR, EMBL/SwissProt, Pearson (Fasta), GDE, Clustal, GCG/MSF,                RSF.

Enter the name of the sequence file :

我输入了我的fasta格式的序列文件名,回车,出现:

Sequence format is Pearson

**************************************************************
******** CLUSTAL 2.1 Multiple Sequence Alignments  ********
**************************************************************

   1. Sequence Input From Disc
   2. Multiple Alignments
   3. Profile / Structure Alignments
   4. Phylogenetic trees

   S. Execute a system command
   H. HELP
   X. EXIT (leave program)

Your choice:
我选择2,回车,出现:
****** MULTIPLE ALIGNMENT MENU ******

1.  Do complete multiple alignment now Slow/Accurate
2.  Produce guide tree file only
3.  Do alignment using old guide tree file

4.  Toggle Slow/Fast pairwise alignments = SLOW

5.  Pairwise alignment parameters
6.  Multiple alignment parameters

7.  Reset gaps before alignment? = OFF
8.  Toggle screen display       = ON
9.  Output format options
I. Iteration = NONE

S.  Execute a system command
H.  HELP
or press [RETURN] to go back to main menu

Your choice:
我输入1,回车,结果1被删除,程序不运行,试了无数次都是这样,哪位知道这是为什么呀?谢谢了!

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分类：基因家族分析

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1 个回答

omicsgene - 生物信息 2020-01-10 09:30

擅长：重测序,遗传进化,转录组,GWAS

检查你输入的序列里面的的ID是否有重复的，如果有重复的会出现输入不进去。

参考：https://www.omicsclass.com/question/1621

clustalw2多序列比对，序列输入不进去

1 个回答

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