1、PAF格式
paf格式为minimap2默认输出格式,结果至少包括12列:
| Col | Type | Description |
| 1 | string | Query序列ID |
| 2 | int | Query序列长度 |
| 3 | int | Query比对开始位置(*based) |
| 4 | int | Query比对结束位置(*based) |
| 5 | char | 如果query/target是正链关系,’+’表示;负链关系,’-‘表示 |
| 6 | string | Target序列ID |
| 7 | int | Target序列长度 |
| 8 | int | Target比对开始位置 |
| 9 | int | Target结束开始位置 |
| 10 | int | 比对上的碱基数 (matching bases) |
| 11 | int | 对齐区域长度(包括gaps) |
| 12 | int | 比对质量(0-255) |
标签说明:
| Tag | Type | Description |
| tp | A | aln类型:P/primary, S/secondary and I,i/inversion |
| cm | i | Number of minimizers on the chain |
| s1 | i | Chaining score |
| s2 | i | Chaining score of the best secondary chain |
| NM | i | Total number of mismatches and gaps in the alignment |
| MD | Z | To generate the ref sequence in the alignment |
| AS | i | DP alignment score |
| SA | Z | List of other supplementary alignments |
| ms | i | DP score of the max scoring segment in the alignment |
| nn | i | Number of ambiguous bases in the alignment |
| ts | A | Transcript strand (splice mode only) |
| cg | Z | CIGAR string (only in PAF) |
| cs | Z | Difference string |
| dv | f | Approximate per-base sequence divergence |
| de | f | Gap-compressed per-base sequence divergence |
| rl | i | Length of query regions harboring repetitive seeds |
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