找到约 15 条结果

文章 HMMER ——hmmsearch 用法,参数

...lled profile hidden Markov models (profile HMMs). HMMER is often used together with a profile database, such as Pfam or many of the databases that participate in Interpro. But HMMER can also work with query sequences, not just profiles, just like BLAST. For example, you can search a protein query ...

问题 fastqc质控时报错,提示Ran out of data in the middle of a fastq entry.是什么问题?

文章 bedtools统计序列中碱基含量

...: Tool:    bedtools nuc (aka nucBed)Version: v2.25.0Summary: Profiles the nucleotide content of intervals in a fasta file.Usage:   bedtools nuc [OPTIONS] -fi <fasta> -bed <bed/gff/vcf>Options:        -fi     Input FASTA file        -bed    BED/GFF/VCF file of ranges to ...

文章 heatmap_group_plot.r 分组热图展示免疫侵润结果

...t  -i filepath, --input filepath                        input the immune res data,[required]  -m filepath, --metadata filepath                        input metadata file path[required]  -g group [group ...], --group group [group ...]                        inpu...

文章 Rocks reinstall on compute node, after disk rebuild, fails

...nk on key tidbit of information would help you here. You only run insert-ethers when you are trying to install a NEW appliance (e.g. compute node). If you just want to re-install an existing node, you do not use insert-ethers. Insert-ethers is a tool to intercept PXE boots, detect MAC addresses, ...

问题 老师您好,进行比较基因组分析时候,单拷贝直系同源群为0,后续的分析就出现了报错,这是什么原因,是我选择的基因组不合适还是其他原因。

...re in orthogroups with 27 or more genes (G50 was 27) and were contained in the largest 6049 orthogroups (O50 was 6049). There were 2129 orthogroups with all species present and 0 of these consisted entirely of single-copy genes. 2025-08-08 15:16:25  2025-08-08 15:16:25 2025-08-08 15:16:25 : Done ...

文章 blast报错

...复制粘贴官网的代码并执行: makeblastdb –in mydb.fsa –dbtype nucl –parse_seqids 总会有如下错误提示: USAGE  makeblastdb [-h] [-help] [-in input_file] [-input_type type]    -dbtype molecule_type [-title database_title] [-parse_seqids]    [-hash_index] [-mask_data m...

问题 circos绘制基因组共线性图绘图命令运行问题

...rence/best_practices   The debugging facility is helpful to figure out what's happening under the   hood       http://www.circos.ca/documentation/tutorials/configuration/debugging   If you're still stumped, get support in the Circos Google Group.       http://groups.google.com/...

问题 转录组KEGG富集分析出现问题。

... normal_vs_tumor --pvalueCutoff 1 --ann.db org.Hs.eg.db --organism hsa --idtype ENSEMBL --totype ENTREZID 输入标黄代码后,出现如下错误: [root@bdc6beb897b9  16:59:07 /work/zhaoran/6.enrich]#Rscript $scriptdir/enrichKEGG_pip.R --deg.file $workdir/5.deg/normal_vs_tumor.DEG.final.tsv ...

问题 RNA 速率分析Pseudotime vs latent time

...enerates a metric known as latent time. I am having a very had time seeing the difference between latent time and standard pseudotime analysis (usually carried out by slingshot, monocle 3, or PAGA) and whether one of them is redundant or not.

问题 您好!我是R语言的新手,在用hchinamap程序绘制地图时,出现cannot coerce type 'closure' to vector of type 'character',出现这类错误怎样解决呢?请指教,谢谢!

步骤如下: install.packages('hchinamap') download.file('https://mdniceczx.oss-cn-beijing.aliyuncs.com/chinadf.rda', file.path(dir, 'chinadf.rda')) load(file.path(dir, 'chinadf.rda'), verbose = TRUE)chinadf

文章 人类癌症数据库突变注释

cosmic70: the latest COSMIC database with somatic mutations from cancer and the frequency of occurence in each subtype of cancer. For more updated cosmic, see instructions below on how to make them.icgc21: International Cancer Genome Consortium version 21 mutations.nci60: NCI-60 human tumor cell lin...

文章 Monocle2|单细胞测序的拟时序分析

...单细胞测序文章《Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer》(IF=14.92)就多次采用拟时序分析,探讨不同细胞亚型之间的分化关系。 拟时序分析 为了方便大家能高效快捷的对单细胞数...

问题 GO注释:blast了nr数据库并通过blast2go获得了GO注释号;也通过blast了swiss-prot数据库获得了GO注释号。这两个结果需要合并吗?

...espectively, were subjected to GO analysis based on sequence alignment and the results were used in GO enrichment analysis. In total, 261 (C. exilicauda) and 197 (M. martensii) CDSs were assigned with 1562 (C. exilicauda) and 1390 (M. martensii) GO terms of knownfunction. The percentages of CDSs wit...

文章 multi_cox.r 基因表达量做多因素cox分析

...e [predict.time ...]                        Time point to draw the ROC curve [default 365 1095                        1825]  -c cut.score, --cut.score cut.score                        set cut score value to divide high and low risk groups                ...