找到约 15 条结果

问题 edger差异分析,如何查询分组差异的结果

...LSE]##归一化TMM y <- calcNormFactors(y,method = 'TMM')#差异分析 #design <- model.matrix(~groups) ###计算离散度,估计离散值(Dispersion) ##估算方法1 #y <- estimateDisp(y,design)####一次性计算common dispersion和tagwise disperson ##估算方法2, y <- estimate...

文章 WRKY基因家族顺势作用原件展示挑选

...e responsiveness; TC-rich repeats, cis-acting element involved in defense and stress responsiveness; ABRE, cis-acting element involved in the abscisic acid responsiveness; circadian, cis-acting regulatory element involved in circadian control; MBS, MYB binding site involved in drought-inducibilit...

问题 差异基因用p-vaule筛选可以吗?

...答。 Point 6: - Line 600: DEGs were determined using p-value < 0.05 and fold change ≥ 1.5. What is the rationale behind using pvalues instead of adjusted pvalues/FDR. Multiple testing correction is a very crucial aspect of RNA-Seq data analysis since there are thousands of genes being tested...

问题 重测序BWA比对率低

...ad2 17205178 + 0 properly paired (44.94% : N/A) 18888798 + 0 with itself and mate mapped 1437966 + 0 singletons (3.76% : N/A) 1344176 + 0 with mate mapped to a different chr 923040 + 0 with mate mapped to a different chr (mapQ>=5) 36840465 + 0 in total (QC-passed reads + QC-failed reads)3...

文章 NCBI的SRA Lite和SRA Normalized数据有什么区别

...ssing overall read quality, setting a per-read quality flag (Read_Filter), and removing base quality scores from the file. In the resulting files, all reads have a Read_Filter flag with value pass or reject. Importantly, it is still possible to produce fastq formatted files from SRA Lite format usin...

文章 植物单细胞转录组文章

...own in gel conditions, 2 wilde type grown in non-compacted soil conditions and 2 wild type grown in compacted soil conditions. scRNA-seq of 2 kitaake grown in gel conditions, 2 Kitaake grown in non-compacted soil conditions. 单细胞转录组揭示玉米根的热胁迫反应机制 Wang, ...

文章 R语言基础入门—数据结构(2)

...ingsAsFactors = FALSE)# Add the "dept" coulmn. emp.data$dept <- c("IT","Operations","IT","HR","Finance") v <- emp.dataprint(v) 当我们执行上面的代码,它产生以下结果 - emp_id emp_name salary start_date dept 1 1 Rick 623.30 2012-01-01 IT ...

文章 iTOL对进化树添加外部标签

...e node branch, or outside the tree#lines starting with a hash are comments and ignored during parsing#=================================================================##                    MANDATORY SETTINGS                           ##=========================================...

文章 MEGAN 分类学树状图 饼图说明

 Each circle of the MEGAN tree represents a taxon in the NCBI taxonomy and is labeled by its name and the number of reads that were assigned to the taxon and not to a subtaxon. The size of the circle is scaled logarithmically to represent the number of reads.  图中圆圈大小代表该物...

文章 GEO数据芯片数据挖掘-在线工具分析GDS数据

...sis Tools 可以查看数据整体的分布情况,例如点击Experiment design and value distribution,可以出现数据box分布图,如下: 单击图片位置,可以查看整个GDS所有样品的数据分布情况,同时查看样品到处理信息(如下图)。 总结 针对...

文章 NCBI批量下载数据,省时又省力

...   os.mkdir(args.out_dir)    dout=os.path.abspath(args.out_dir)output_handle = open(dout+'/'+args.name+'.%s'%args.rettype, "w")Entrez.email = "huangls@biomics.com.cn"     # Always tell NCBI who you are#handle = Entrez.efetch(db="nucleotide", id="EU490707", rettype="gb", retmode="text")#print(h...

文章 lasso_cox.r lasso_cox 模型优化分析

...alysis optional arguments: -h, --help show this help message and exit -i data, --data data input data file path[required] -t time, --time time set suvival time column name [required] -e event, --event event set event column name [required] -v variate [...

文章 R安装units包报错解决方法

...ource* package ‘units’ ...** package ‘units’ successfully unpacked and MD5 sums checked** using staged installationconfigure: units: 0.8-2checking whether the C++ compiler works... yeschecking for C++ compiler default output file name... a.outchecking for suffix of executables...checking whe...

问题 RNAseq有参转录组数据自主分析课程,在建立基因组索引的时候出现如下问题

...务器上运行,最后获取gene length时候出错: get gene length and gene.bed from gtf: RUN CMD: python /home/zpp/work/my_rnaseq/scripts/get_gene_length_from_gtf.py -g Homo_sapiens.GRCh38.99.chromosome.22.gtf -p gene_length Traceback (most recent call last):   File "/home/zpp/work/my_rn...

文章 芯片差异分析分组矩阵

...数完成,获得的分组矩阵结构类似下方显示结果: > design           A BGSM1626001 1 0GSM1626002 1 0GSM1626003 1 0GSM1626010 0 1GSM1626011 0 1GSM1626012 0 1attr(,"assign")[1] 1 1attr(,"contrasts")attr(,"contrasts")$`description`[1] "contr.treatment" ...