找到约 15 条结果

问题 plink报错

...您好  我在用plink将vcf转tped格式的时候出现了报错Error: .bim file has a split chromosome.  Use --make-bed by itself to remedy this. 添加--make-bed后依旧没有解决  命令: plink --vcf SV.vcf --maf 0.01 --geno 0.5 --recode 12 transpose --out SV_filter --set-missing-var-id...

问题 asv分类报错

...ed between creating '/home/wy/rawdata/am-test/data/my_ampliseq/tmp/qiime2' and setting permissions on it.The above exception was the direct cause of the following exception:Traceback (most recent call last):  File "/root/miniconda3/envs/qiime2-amplicon-2024.2/lib/python3.8/site-packages/q2cli/click...

问题 edger差异分析,如何查询分组差异的结果

...LSE]##归一化TMM y <- calcNormFactors(y,method = 'TMM')#差异分析 #design <- model.matrix(~groups) ###计算离散度,估计离散值(Dispersion) ##估算方法1 #y <- estimateDisp(y,design)####一次性计算common dispersion和tagwise disperson ##估算方法2, y <- estimate...

文章 MUMmer + SVMU : CNVs

...######################################### SVMU to produce collinear blocks and insertions, deletions and CNVs.##### 由于 lastz 太慢,因此多样本 批量运行if [ -f lastz.cmds.sh ] ; then rm -f lastz.cmds.shfiif [ -f svmu.cmds.sh ] ; then rm -f svmu.cmds.shfi#批量产出命令for query i...

文章 cactus-bin-v2.9.9 安装笔记

...distribution.## ExtractingIf you have not already extract the distribution and cd into the cactus directory:```tar -xzf cactus-bin-v2.9.9.tar.gzcd cactus-bin-v2.9.9```## GPU Patch:When using GPU support since v2.9.4, please make sure to pass in the patched config file with `--configFile` (download t...

文章 WRKY基因家族顺势作用原件展示挑选

...e responsiveness; TC-rich repeats, cis-acting element involved in defense and stress responsiveness; ABRE, cis-acting element involved in the abscisic acid responsiveness; circadian, cis-acting regulatory element involved in circadian control; MBS, MYB binding site involved in drought-inducibilit...

文章 R语言基础入门—数据结构(2)

...ingsAsFactors = FALSE)# Add the "dept" coulmn. emp.data$dept <- c("IT","Operations","IT","HR","Finance") v <- emp.dataprint(v) 当我们执行上面的代码,它产生以下结果 - emp_id emp_name salary start_date dept 1 1 Rick 623.30 2012-01-01 IT ...

问题 差异基因用p-vaule筛选可以吗?

...答。 Point 6: - Line 600: DEGs were determined using p-value < 0.05 and fold change ≥ 1.5. What is the rationale behind using pvalues instead of adjusted pvalues/FDR. Multiple testing correction is a very crucial aspect of RNA-Seq data analysis since there are thousands of genes being tested...

文章 NCBI的SRA Lite和SRA Normalized数据有什么区别

...ssing overall read quality, setting a per-read quality flag (Read_Filter), and removing base quality scores from the file. In the resulting files, all reads have a Read_Filter flag with value pass or reject. Importantly, it is still possible to produce fastq formatted files from SRA Lite format usin...

问题 重测序BWA比对率低

...ad2 17205178 + 0 properly paired (44.94% : N/A) 18888798 + 0 with itself and mate mapped 1437966 + 0 singletons (3.76% : N/A) 1344176 + 0 with mate mapped to a different chr 923040 + 0 with mate mapped to a different chr (mapQ>=5) 36840465 + 0 in total (QC-passed reads + QC-failed reads)3...

文章 iTOL对进化树添加外部标签

...e node branch, or outside the tree#lines starting with a hash are comments and ignored during parsing#=================================================================##                    MANDATORY SETTINGS                           ##=========================================...

文章 GEO数据芯片数据挖掘-在线工具分析GDS数据

...sis Tools 可以查看数据整体的分布情况,例如点击Experiment design and value distribution,可以出现数据box分布图,如下: 单击图片位置,可以查看整个GDS所有样品的数据分布情况,同时查看样品到处理信息(如下图)。 总结 针对...

文章 MEGAN 分类学树状图 饼图说明

 Each circle of the MEGAN tree represents a taxon in the NCBI taxonomy and is labeled by its name and the number of reads that were assigned to the taxon and not to a subtaxon. The size of the circle is scaled logarithmically to represent the number of reads.  图中圆圈大小代表该物...

文章 NCBI批量下载数据,省时又省力

...   os.mkdir(args.out_dir)    dout=os.path.abspath(args.out_dir)output_handle = open(dout+'/'+args.name+'.%s'%args.rettype, "w")Entrez.email = "huangls@biomics.com.cn"     # Always tell NCBI who you are#handle = Entrez.efetch(db="nucleotide", id="EU490707", rettype="gb", retmode="text")#print(h...

文章 lasso_cox.r lasso_cox 模型优化分析

...alysis optional arguments: -h, --help show this help message and exit -i data, --data data input data file path[required] -t time, --time time set suvival time column name [required] -e event, --event event set event column name [required] -v variate [...