找到约 15 条结果

文章 perl脚本批量生成反向互补序列

...  must be given-fa fasta file <infile> must be givenOther parameter:-h           Help documentUsage End.exit;}my $in  = Bio::SeqIO->new(-file => "$opts{fa}" , -format => 'Fasta');open(OUT,">$opts{out}") ||die "open file $opts{out} faild.\n";while ( my $seq = $i...

问题 Perl GD模块官网例子报错

...e(255,0,0);       $blue = $im->colorAllocate(0,0,255);   # make the background transparent and interlaced $im->transparent($white); $im->interlaced('true');   # Put a black frame around the picture $im->rectangle(0,0,99,99,$black);   # Draw a blue oval $im->arc(50,50...

文章 超大基因组，超长染色体，凤丹牡丹基因组怎一个难字了得！

...Nature Communications（IF=17.69）上发表了题为“Genomic basis of the giga-chromosomes and giga-genome of tree peony Paeonia ostii”的研究论文。该研究公布了凤丹牡丹的高质量参考基因组，解析了凤丹牡丹巨大基因组的形成与维持机制；结合448份材料...

问题 用TBtools绘制两个物种的进化树+motif+基因结构三连图时报错问题

...used as input do       NOT present in gff/gtf file.      Examples of valid IDs is showed as bellowedGuess FileType is:GTFtranscript:AT1G03987.1transcript:AT1G01020.6transcript:AT1G01010.1transcript:AT1G01020.3transcript:AT1G01020.2transcript:AT1G01020.5transcript:AT1G01020.4transcript:AT1G0...

文章 从基因组注释信息GFF文件中提取所有基因位置信息-AWK

... $ grep -v '#' Arabidopsis_thaliana.TAIR10.41.gff3|awk -F "[\t=:;]" 'BEGIN{OFS="\t"}$3=="gene"{print $1,$4,$5,$10}' |head1       3631    5899    AT1G010101       6788    9130    AT1G010201       11649   13714   AT1G010301       23121   31227   AT1G010401       311...

文章 R包 CandiHap 进行单倍型分析

...iHap)dat <- read_data("track-Phenotype.gwas.txt",sep="\t")# notice that the dat should have the chromosome name in the first column, position in the second column and the values in the following columns snptrack(gff,dat=dat,id="Parent",color='r2', point.size = 0.2, high = "#DC0000B2",low ="blue"...

问题 在初次docker运行程序时发现问题

WARNING: The requested image's platform (linux/amd64) does not match the detected host platform (linux/arm64/v8) and no specific platform was requested

文章 必须收藏！比较基因组学分析必备神器-OrthoVenn2

...d annotation oforthologous clusters across multiple species, Nucleic Acids Research, Volume 47, Issue W1, 02 July 2019, Pages W52–W58, https://doi.org/10.1093/nar/gkz333. 日常科研中你我经会常遇到看不懂的图表，不会挖掘的数据，没有思路的文章，沟通不畅的个性...

文章 转录组分析基因注释常用的数据库

...注释的蛋白数量不多。4、TrEMBL：数据库全称“Translation of EMBL”，是从EMBL中的cDNA序列翻译得到的，其中TrEMBL收录的是未经人工注释的编码DNA序列翻译数据。(unreviewed, automatically annotated)，不难看出，相比之下，sp数据库更可靠。 ...

文章 TCGA 数据的多组学WGCNA联合分析

...lncRNA, mRNA， miRNA 等，而文章《identifying mirna and gene modules of colon cancer associated with pathological stage by weighted gene co-expression network analysis》则结合Gene,miRNA 分别进行WGCNA的分析，获得性状相关的Hub Gene和 Hub miRNA, 再基于miRNA能调控Gene 的...

文章 外源插入位置查找好方法

...言。 参考文献： Esher SK, Granek JA, Alspaugh JA. Rapid mapping of insertional mutations to probe cell wall regulation in Cryptococcus neoformans. Fungal genetics and biology : FG & B. 2015;82:9-21. doi:10.1016/j.fgb.2015.06.003.

文章 实验室linux生物信息服务器搭建CentOS7

...a Cloud Computing http://mirrors.aliyun.com/centos/ Beijing Institute of Technology http://mirror.bit.edu.cn/centos/ ChongQing University http://mirrors.cqu.edu.cn/CentOS/ CN99 Corp. http://mirrors.cn99.com/centos/ Dalian Neusoft University of Information http://mirrors.neusoft.edu.c...

问题 请问下jupyter notebook运行一个不到5M(20万行左右)的文件，内存持续飙升，随后浏览器闪退该怎么解决？

...好，内存16G, amd，i7处理器，win11系统，联想笔记本R9000X 2021R。

问题 Picard sortsam排序

...-Line-Syntax-Transition-For-Users-(Pre-Transition) ********** ********** The command line looks like this in the new syntax:

问题 GeMoMa报错

...报错java.lang.IllegalArgumentException: You selected cdsParts=true, but the ID (gene-si:)seems to be no CDS part.Exception in thread "main" java.lang.RuntimeException: Did not finish as intended. java.lang.IllegalArgumentException: You selected cdsParts=true, but the ID (gene-si:)seems to be no CD...