60 用TBtools绘制两个物种的进化树+motif+基因结构三连图时报错问题

物种是拟南芥和水稻;

使用单个物种(拟南芥)时绘图正常;我把二者的GTF文件合并想一起绘图,却出现了一下错误:

=========================================
==============A Big Warniing=============
=========================================
 IDs that you used as input do      
 NOT present in gff/gtf file.     
 Examples of valid IDs is showed as bellowed
Guess FileType is:GTF
transcript:AT1G03987.1
transcript:AT1G01020.6
transcript:AT1G01010.1
transcript:AT1G01020.3
transcript:AT1G01020.2
transcript:AT1G01020.5
transcript:AT1G01020.4
transcript:AT1G01020.1
transcript:AT1G01030.2
transcript:AT1G01030.1
transcript:AT1G01040.1
=========================================
六月 21, 2020 10:39:33 上午 biocjava.bioDoer.MEME.DrawMotifPattern.DrawGeneStructureFromGXFfile metaParser
严重: null
java.io.IOException: Parsing Gxf Error...See above...
at biocjava.bioDoer.MEME.DrawMotifPattern.DrawGeneStructureFromGXFfile.metaParser(DrawGeneStructureFromGXFfile.java:87)
at biocjava.bioDoer.MEME.DrawMotifPattern.DrawMotifPatternFromMEMEResult.postGraph(DrawMotifPatternFromMEMEResult.java:1020)
at biocjava.bioDoer.MEME.DrawMotifPattern.DrawAmazingMetaPlot.plot(DrawAmazingMetaPlot.java:617)
at biocjava.GUIexcutors.JIGplotToolkit.AmazingGeneViewGUIPanel$15$1.run(AmazingGeneViewGUIPanel.java:490)
六月 21, 2020 10:39:33 上午 biocjava.GUIexcutors.JIGplotToolkit.AmazingGeneViewGUIPanel$15$1 run
严重: null
java.lang.NullPointerException
at biocjava.bioDoer.MEME.DrawMotifPattern.DrawMotifPatternFromMEMEResult.postGraph(DrawMotifPatternFromMEMEResult.java:1029)
at biocjava.bioDoer.MEME.DrawMotifPattern.DrawAmazingMetaPlot.plot(DrawAmazingMetaPlot.java:617)
at biocjava.GUIexcutors.JIGplotToolkit.AmazingGeneViewGUIPanel$15$1.run(AmazingGeneViewGUIPanel.java:490)
这个是进化树文件:
attachments-2020-06-7wPYiUyO5eeec8b803e3f.png
这个是合并后的GTF文件交界处(不知道能不能这样用?):
attachments-2020-06-wHV7gGsK5eeec8dbeedfb.png
------------------------------------------

绘制 进化树+motif 二连图则正常。。。


(更新:进化树+motif 二连图)

attachments-2020-06-19bLgcNf5ef07cd64096e.png

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最佳答案 2020-06-22 21:20

解决了,原来是没删去引号里的“transcript:” 汗.....

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其它 1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

你的基因组注释文件GFF是不是弄错了,既有拟南芥的基因,又有水稻的基因;

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