那位大神能帮忙看一下嘛,failed to open SRA manager是怎么回事?
fastq-dump.2.8.0 err: path not found while searching dynamic library within file system module - failed to open SRA manager
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An error occurred during pro...
The experimentally verified miRNA-mRNA pairs were downloaded from starBase v3.0 (18), DIANA-Tarbase v8 (19), miRTarbase (v7.0) (20) and miRSponge (21) databases, while the experimentally validated miRNA-lncRNA pairs were extracted from starBase v3.0 (18), LncACTdb2.0 (22), DIANA-LncBase Experimental...
..._hba.conf entry for host "::1", user "drupaladmin", database "sitedb", SSL off in Drupal\Component\DependencyInjection\PhpArrayContainer->createService() (line 79 of /var/www/drupal8/web/core/lib/Drupal/Component/DependencyInjection/PhpArrayContainer.php).Drupal\Component\DependencyInjection\PhpA...
...起来就相对的比较模糊了,官方的定义是e-value:the number of hits you expect to see by chance/false positive .
对于定义里面的“by chance”,也就是随机情况下指的是什么意思不是很了解,随后查阅了一下文献,是这样解释的:
by chance means...
...and:
(1/1) Invalid value for '--i-reference-seqs': Expected an artifact of at
least type FeatureData[Sequence]. An artifact of type TaxonomicClassifier
was provided.
老师,这是原始脚本,ITS的 和18S的'--i-reference-seqs' 都找不到
There was a problem importing /work/omicsclass/ampliseq-q2/qiime2_2022.8/data_2/manifest.ckd.txt:
/work/omicsclass/ampliseq-q2/qiime2_2022.8/data_2/manifest.ckd.txt is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 3 and column "forward-absolute-filepath" could not be fo...
...rnaseq/125PS_125NS/ref/gene.bed -o DL125NS1_inner_size
Get exon regions from /work/zhaoran_rnaseq/125PS_125NS/ref/gene.bed ...
Load BAM file ... Done
Total read pairs used 0
Cannot find paired reads
RUN CMD: inner_distance.py -i /work/zhaoran_rnaseq/125PS_125NS/3.map/hisat2/DL125NS2.bam...
... 119, in load_vcf_wrapper
assert "samples" in callset.keys(), "None of the samples provided in {0!r} are found in {1!r}".format(
AttributeError: 'NoneType' object has no attribute 'keys'