找到约 15 条结果

问题 在运行core_pan_gene_curve.r的时候报错

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Attaching package: ‘dplyr’ The following objects are masked from ‘package:s...

问题 TCGAbiolinks GDCprepare使用问题 提示找不到文件?

... data.category = "Transcriptome Profiling", data.type = "Gene Expression Quantification", workflow.type = "HTSeq - Counts") GDCdownload(query) data <- GDCprepare(query) 下载成功了,自动生成了GDCdata文件夹,但它里面是空的,...

文章 碰到奇葩审稿人怎么办?这位作者靠怒怼审稿人登上SCI期刊!

...,到底如何来应对? 学术界有句话叫: “It alldepends on the reviewers(一切都取决于审稿人)” 上面那位作者的结局虽然是好的,但是我们并不建议你也这么做, 毕竟,你怼人的“功力”,可能完全没有那些摸爬滚打很多年...

文章 samtools使用

...ls都有哪些命令 $samtools Program: samtools (Tools for alignments in the SAM format) Version: 1.9 (using htslib 1.9) Usage: samtools <command> [options] Commands: -- Indexing dict create a sequence dictionary file faidx index/extract FASTA fqidx ...

问题 有参转录组分析

...rieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") :   cannot open file 'NA...

文章 ggalluvial 展示ceRNA调控关系

...可视化每个基因的连接程度。 参考文献 Construction and comprehensive analysis of a ceRNA network to reveal potential prognostic biomarkers for hepatocellular carcinoma.

文章 NCBI下载的10X单细胞数据只有read1和read2如何读入cellranger

...7.0/using/fastq-input#wrongname  [Sample Name]_S1_L00[Lane Number]_[Read Type]_001.fastq.gz # 其中Read Type# I1: Sample index read (optional)# R1: Read 1# R2: Read 2 NCBI下载的数据,为双端reads,不符合cellranger mkfastq出来的命名规则,后续cellranger count 无法读入,...

问题 绘制circos图,途中不显示共线性基因

...g directory /home/manager/GeneFA/sharegb/cir debuggroup summary 0.97s command /biosoft/circos/circos-0.69-6/bin/circos -conf /home/manager/GeneFA/sharegb/cir/config1.txt -outputdir /home/manager/GeneFA/sharegb/cir/result1 -outputfile test1 debuggroup summary 0.97s loading configuration from file /...

问题 老师们好,在生成gvcf文件合并vcf文件时,出现这种警告信息,请问是什么原因,如何处理啊?

...ffold_1 position 9583478 (TileDB column 9583477) has too many genotypes in the combined VCF record : 1081 : current limit : 1024 (num_alleles, ploidy) = (46, 2). Fields, such as  PL, with length equal to the number of genotypes will NOT be added for this sample for this locatio

文章 转录组测序分析水稻旱作的重要基因

...hmanyam, M. B. B. Prasad Babu & Polumetla A. Kumar RNA-seq reveals the involvement of key genes for aerobic adaptation in rice. Scientific Reports volume 9, Article number: 5235 (2019) 推荐: 二代测序转录组数据自主分,转录组标准分析后的数据挖掘、转录组文献...

文章 4个样品重测序发PlosOne

...高深度重测序,四个品种中三个高抗白粉病( Edisto, MR-1, and PMR5: resistant),一个易感白粉病(SCNU1154: susceptible;),得到测序reads与参考基因组比对,查找SNP,Indel,SV等变异信息,并对变异信息进行质控过滤,变异信息在染色体...

文章 基因家族分析如何能发到cells?!

... ntegrative Analysis of the Wheat PHT1 Gene Family Reveals A Novel Member Involved in Arbuscular Mycorrhizal Phosphate Transport and Immunity. Yi Zhang at all. Cells, 2019 05 22; 8(5) . 总有同学咨询基因家族分析类文章可以投哪些期刊,闲暇之余,小编就整理了一...

文章 circos当中的颜色介绍

...字符来表示颜色,常见的:red, orange, yellow, green, blue, and purple等,可在这些颜色前面加上修饰,变换颜色的深浅:例如vvlred  表示:very very light version of red vvl{name} - very very light version of color vl{name} - very light l{name} - light {nam...

文章 endnote 如何插入书籍引用

...索到,google books。例如我要引用 这本书里面《Biosynthesis and perception of melon aroma》关于甜瓜香气的章节(281–305页):谷歌学术搜索第一个是一本书, 这里没有文献citation下载: 但是回到谷歌搜索的结果会有两个版本: ...

文章 cutree对pheatmap返回结果实现聚类cluster划分

...and labels, of appropriate content each.kan integer scalar or vector with the desired number of groupshnumeric scalar or vector with heights where the tree should be cut. 所以将热图结果中的list$tree_row作为tree输入即可: list=pheatmap(mat,scale = "row")row_cluster=cutree(list$...