找到约 15 条结果

问题 合并选择区域时出现错误,请教一下,要怎么解决呢?是不是把前面的- 删掉

...i - >fst_top0.5_selected_region.txt Error: Sorted input specified, but the file - has the following out of order record hic_scaffold_13100014100002.170817908590890.004903580.00525442 -0.0996961085700556FS_D selected region

问题 群体遗传学GWAS课程docker很多命令无法运行

...lter/all.varFilter.vcf.gz -SubPop popid.txt -MaxDist 500 -OutStat ld.stat the Number of subPop samples[found in VCF] is 0 sub Group Population szie is too small, SubGroup sample size: 0 ##begin pair-wise R^2 cal. after filter Remain SNP Number :     0 #% number bin is        1 1%......--...

文章 barplot()R语言绘制状图

...101467081Amino Acid Metabolism0.0990784610.1023070980.098133001Replication and Repair0.0640276080.081496790.077107142Energy Metabolism0.0630605790.0669996830.055660175Translation0.0486662250.0525558320.048639947Poorly Characterized0.0501483020.0474659650.049391479Metabolism of Cofactors and Vitamins...

问题 在提取1500bp序列做顺式作用元件检测时,使用tiquxulie.pl这个脚本,开始时显示out of memory,我把虚拟内存提高到了4G,显示zsh:killed,得到的结果还是空的,请问老师这个问题怎么解决呢?

谢谢老师。

问题 TCGA下载数据保存

下载FPKM的时候,保存结果时出现Error in read_connection_(con) : Evaluation error: error reading from the connection. In addition: Warning message: In (function (con, what, n = 1L, size = NA_integer_, signed = TRUE,  :   invalid or incomplete compressed data

问题 用Tassel转vcf为hapmap格式文件

...1B  Pos:711831      Name:S1B_711831 Variants:C/T    MAF:NaN Ref:C and Position      Chr:7A  Pos:736691630   Name:S7A_736691630  Variants:T/C     MAF:NaN Ref:T out of order.

文章 BSA分析之MutMap分析原理详解!

...tructed by replacing nucleotides in Nipponbare with those of Hitomebore at the 124,968 SNP positions identified between the two cultivars by alignment of 12.25 Gb of Illumina Hitomebore short reads to the Nipponbare reference genome MutMap适用条件: 1. 一般为隐性突变,最好是EMS诱...

问题 在运行core_pan_gene_curve.r的时候报错

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Attaching package: ‘dplyr’ The following objects are masked from ‘package:s...

问题 TCGAbiolinks GDCprepare使用问题 提示找不到文件?

... data.category = "Transcriptome Profiling", data.type = "Gene Expression Quantification", workflow.type = "HTSeq - Counts") GDCdownload(query) data <- GDCprepare(query) 下载成功了,自动生成了GDCdata文件夹,但它里面是空的,...

问题 有参转录组分析

...rieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") :   cannot open file 'NA...

文章 碰到奇葩审稿人怎么办?这位作者靠怒怼审稿人登上SCI期刊!

...,到底如何来应对? 学术界有句话叫: “It alldepends on the reviewers(一切都取决于审稿人)” 上面那位作者的结局虽然是好的,但是我们并不建议你也这么做, 毕竟,你怼人的“功力”,可能完全没有那些摸爬滚打很多年...

文章 samtools使用

...ls都有哪些命令 $samtools Program: samtools (Tools for alignments in the SAM format) Version: 1.9 (using htslib 1.9) Usage: samtools <command> [options] Commands: -- Indexing dict create a sequence dictionary file faidx index/extract FASTA fqidx ...

文章 ggalluvial 展示ceRNA调控关系

...可视化每个基因的连接程度。 参考文献 Construction and comprehensive analysis of a ceRNA network to reveal potential prognostic biomarkers for hepatocellular carcinoma.

文章 NCBI下载的10X单细胞数据只有read1和read2如何读入cellranger

...7.0/using/fastq-input#wrongname  [Sample Name]_S1_L00[Lane Number]_[Read Type]_001.fastq.gz # 其中Read Type# I1: Sample index read (optional)# R1: Read 1# R2: Read 2 NCBI下载的数据,为双端reads,不符合cellranger mkfastq出来的命名规则,后续cellranger count 无法读入,...

文章 转录组测序分析水稻旱作的重要基因

...hmanyam, M. B. B. Prasad Babu & Polumetla A. Kumar RNA-seq reveals the involvement of key genes for aerobic adaptation in rice. Scientific Reports volume 9, Article number: 5235 (2019) 推荐: 二代测序转录组数据自主分,转录组标准分析后的数据挖掘、转录组文献...