Mouse breeding
Tissue processing and library construction for scRNA-seq（咨询测序公司）
scRNA-seq data pre-processing
Preprocessing of raw data was initially performed using Cell Ranger software pipeline (version 9.0.1)(Zheng et al. 2017). This step included reads alignment ...
use strict;
use warnings;
print STDERR "gene symbol column number: ";
my $geneSymbolCol=<STDIN>;
chomp($geneSymbolCol);
$geneSymbolCol--;
my $expFile="probeMatrix.txt";
my $gplFile="ann.txt";
my $expFileWF="geneMatrix.txt";
my %hash=();
my @sampleName=();
open(EXP,"$expFile") or d...
use strict;use warnings;print STDERR "gene symbol column number: ";my $geneSymbolCol=<STDIN>;chomp($geneSymbolCol);$geneSymbolCol--;my $expFile="probeMatrix.txt";my $gplFile="ann.txt";my $expFileWF="geneMatrix.txt";my %hash=();my @sampleName=();open(EXP,"$expFile") or die $!; open(PL,"GEOimmun...