找到约 15 条结果

文章 R magick 包安装包错

...omType = opt$idtype, toType = opt$totype, OrgDb = opt$ann.db) :   26.02% of input gene IDs are fail to map... --> No gene can be mapped.... --> Expected input gene ID: --> return NULL... NULL Error in (function (classes, fdef, mtable)  :   unable to find an inherited method for fu...

问题 win10企业版本,专业版本安装docker失败 WSL 2 installation is incomplete.

问题 下载COAD数据提示列不一致保存不成功

...les.per.chunk=6, method='client') Downloading data for project TCGA-COAD Of the 521 files for download 521 already exist. All samples have been already downloaded > # 保存结果 > data <- GDCprepare(query = query,  +                    save = TRUE,  +             ...

文章 blastn使用教程及结果详解

...进行比对。 ## 下载Blastwget -c ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.10.0+-x64-linux.tar.gz## 解压tar -xvzf ncbi-blast-2.10.0+-x64-linux.tar.gz 二、blastn使用 1.建库 makeblastdb -in genome.fasta -dbtype nucl -parse_seqids -out ./index -in:构...

问题 运行DESeq2出错

各位老师,可以帮我看一下吗 Error in h(simpleError(msg, call)) :   error in evaluating the argument 'x' in selecting a method for function 'rowSums': could not find function "cpm"Calls: std_two_condition_comp_deseq2 -> new_DGE_list -> rowSums -> .handleSimpleError -> h

问题 基因组注释Braker里面的报错

..._broken_genes” 报错“# Mon Aug 12 20:10:06 2024: Log information is stored in file /work/work/xsyj/genome_annotation/03.Braker/braker/braker.log ERROR in file /share/work/biosoft/BRAKER/latest/scripts//braker.pl at line 6666 Failed to execute: perl /share/work/biosoft/GeneMark-ES/latest/gmes...

文章 单细胞RNA速率(RNA velocity)分析结果解读

...确地描绘了细胞分化轨迹。 velocity length: the speed or rate of differentiation is given by the length of the velocity vector.velocity confidence:  the coherence of the vector field (i.e., how a velocity vector correlates with its neighboring velocities) provides a measure of confidence...

问题 安装github上的包报错:

install_github("ricardo-bion/ggradar") Using github PAT from envvar GITHUB_PAT. Use `gitcreds::gitcreds_set()` and unset GITHUB_PAT in .Renviron (or elsewhere) if you want to use the more secure git credential store instead. Error: Failed to install 'unknown package' from GitHub:   HTTP error 40...

问题 使用get_gene_length_from_gtf.py脚本统计基因长度时报错

...件,然后用gffread转换为了gtf文件,然后使用get_gene_length_from_gtf.py 这个python脚本统计,都是教程上的步骤,然后就报错了 报错信息如下 Traceback (most recent call last):   File "../script_new/get_gene_length_from_gtf.py", line 48, in <module>  ...

文章 HISAT2比对时报错|(ERR): hisat2-align died with signal 9 (KILL)

...脚本: hisat2 -p 10 --rg-id=L2 --rg SM:L2 --rg LB:L2 --rg PL:ILLUMINA -x iwgsc_refseqv2.1_assembly --dta -1 L2_1.clean.fq.gz -2 L2_2.clean.fq.gz 2> L2.summary 运行一段时间后,出现报错: (ERR): hisat2-align died with signal 9 (KILL) google之后怀疑是基因组太大,然后...

文章 linux下解压命令

...见类型的文件如何压缩以及解压缩。 .tar  解包:tar xvf FileName.tar 打包:tar cvf FileName.tar DirName 要注意  tar 是打包,而不是压缩哦! .gz 解压1:gunzip FileName.gz 解压2:gzip -d FileName.gz 压缩:gzip FileName .tar.gz 和 .tgz 解压...

文章 用R语言快速合并大量表格文件

...t.files(".",pattern = "*_counts.tsv")datalist = lapply(filenames, function(x){  read.table(file=x,header=T,sep = "\t",check.names = F)})dd=join_all(datalist,by="id", type="full")  #注意指定文件共同的列明by, type 也有left 以第一个文件ID为准,right,inner  取交集,full ...

问题 关于基因芯片affy包rma标准化

看了GEO芯片数据标准化的视频,关于affy包,请问如果后续分析需要未进行log转换数据,那我可不可以对rma处理的数据进行2^x进行反对数转换?还是应该直接用mas5.0标准化?

文章 RIP实验技术

...6).         3.Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes. Nucleic Acids Res(2017).

文章 群体基因交流分析的几个统计值

...ints202003.0237.v2.pdf fb:https://www.nature.com/articles/s41559-018-0717-x Dsuite:https://onlinelibrary.wiley.com/doi/epdf/10.1111/1755-0998.13265