I am doing scRNA-Seq analysis, and I am doing RNA velocity and pseudotime analyses as part of my analysis pipeline. Now, RNA velocity is carried out using scvelo, and this package generates a metric known as latent time. I am having a very had time seeing the difference between latent time and stand...
...ased on the mate info in the BAM R2 and Q2 tags.Tips: If you want to create a single, interleaved FASTQ file for paired-end data, you can just write both to /dev/stdout: bedtools bamtofastq -i ...
...type2 fribidi is already installed, check that 'pkg-config' is in yourPATH and PKG_CONFIG_PATH contains a harfbuzz freetype2 fribidi.pc file. If pkg-configis unavailable you can set INCLUDE_DIR and LIB_DIR manually via:R CMD INSTALL --configure-vars='INCLUDE_DIR=... LIB_DIR=...'---------------------...
...st one zero, cannot compute log geometric means
data frame with 0 columns and 0 rows
Error in `group_by_prepare()`:
! Must group by variables found in `.data`.
• Column `cluster` is not found.
Backtrace:
▆
1. ├─obj.markers %>% group_by(cluster) %>% ...
2. ├─dplyr:...
...元素么?
The any function has the same syntax as , accepting a block and a list of values, but it only returns true or false. True, if the block gives true for any of the values. False if none of them match. It also short circuits so on large lists this can be a lot faster.
any函数的语法...
...面并没有这一项。
Forces EXPR to be interpreted in scalar context and returns the value of EXPR.
中文含义：强制表达式EXPR在标量上下文进行解释。
假如，我定义了一个数组@arr=("one","two","three")，该数组在标量环境下返回自身的长度。
print @a...
...install.packages("WGCNA")
Package which is only available in source form, and may need compilation of C/C++/Fortran:
‘WGCNA’
Do you want to attempt to install these from sources? (Yes/no/cancel) Yes
installing the source package ‘WGCNA’
试开URL’https://cran.rstudio.com/src/cont...
...Long noncoding RNAs AC026904.1 is
essential for TGF-β-induced migration and epithelial-mesenchymal
transition through functioning as an enhancer of Slug in lung cancer
cells”的文章，研究lncRNA
AC026904.1在肺癌转移中的作用。发现AC026904.1在转录水平上调Slug表达，进...
...have new IP addresses. You may need to re-run the `docker-machine env` command.
Regenerate TLS machine certs? Warning: this is irreversible. (y/n): Regenerating TLS certificates
Waiting for SSH to be available...
Detecting the provisioner...
Copying certs to the local machine directory...
Cop...
... vt
2. cd vt
#run make, note that compilers need to support the c++0x standard
3. make
#you can test the build
4. make test
报错1：
gcc -g -Wall -O2 -I. -c -o cram/cram_index.o cram/cram_index.cgcc -g -Wall -O2 -I. -c -o cram/cram_io.o cram/cram_io.ccram/cram_io.c:61:18: error: lzma...