问题 老师你好，构建进化树16S分析一直出错

...个 Plugin error from phylogeny: work/my_amplicon/tmp/q2-AlignedDNAFASTAFormat-3r8bwdoj is not a(n) AlignedDNAFASTAFormat file:   The sequence starting on line 2 was length 404. All previous sequences were length 0. All sequences must be the same length for AlignedFASTAFormat. Debug info ha...

用户 One or Zero

用户 （HM＆LA)or

问题 当保留蛋白编码序列时，为什么会提示有这个报错？检查发现'!'应该是没有输入错误的

原命令：agat_sp_filter_feature_by_attribute_value.pl --gff $gff --attribute biotype --value protein_coding -t '!' -o $species.protein_coding.gff3 报错：The test to apply is Wrong: !. We want something among this list: <,>,<=,>=,! or =.

问题 遗传图谱构建

for chr in  {1..12};do  echo "snpbinner visualize --out bin.visualize.$chr --crosspoints pop.crosspoints.$chr  --snps pop.forbin.$chr --bins  pop.bin.$chr" done >view.sh ParaFly -c view.sh -CPU 5 该步一直运行不出结果，demo中01.PrepareData的bin.visualize.1-12文件夹为...

用户 Is.劉源

用户 Order is

话题 is.na

问题 第一步文件导入qiime2一直出错，按照视频设置好

There was a problem importing /work/my_amplicon/A/manifest.ckd.txt:   /work/my_amplicon/A/manifest.ckd.txt is not a(n) PairedEndFastqManifestPhred33V2 file:   Filepath on line 1 and column "forward-absolute-filepath" could not be found ($datadir/SRR7441413_1.fastq) for sample "SRR7441413".

问题 动植物泛基因组分析实操课程资料问题

...dard /share/work/biosoft/perl/perl-5.22.1/lib/site_perl/5.22.1/auto/share/dist/AGAT/agat_config.yaml file The test to apply is Wrong: !. We want something among this list: <,>,<=,>=,! or =.[），最终导致运行结果是空的

用户 Ma is maize~So

问题 ALLHIC显示找不到sample.bwa_mem.bam

...C1或者2的时候都报错，均有显示[E::idx_find_and_load] Could not retrieve index file for '/work/genome/assembly/output/docker/GM/30.ALLHiC2/sample.bwa_mem.bam'。但该地址下是有该文件的。 第二个问题是ALLHIC1时形成了sample.clean.counts_GATC.13g1.txt、sample.clean.coun...

问题 在docker中用cleandata与宿主序列比对得到sam文件这一步出现错误，

输入的命令是for i in `tail -n+2 $datafile/metadata.txt|cut -f1`;do bowtie2 --threads 6 \ -x $host_index/Canis_lupus/wolf \ -1 $workdir/1.host_del_and_qc/cleandata/${i}_1.fastq \ -2 $workdir/1.host_del_and_qc/cleandata/${i}_2.fastq \ -S $workdir/1.host_del_and_qc/cleandata/${i}.bowtie.sam \ --v...

文章 绘制类似于箱线图的散点图

...rary(ggplot2)library('getopt');spec = matrix(c('help' , 'h', 0, "logical","for help",'input1' , 'i', 1, "character","input the abuance of tax in each sample ,required",'input2' , 's', 1, "character","input the abuance of tax in each sample ,required",#'row' , 'r' , 1 , "character","row name,required...

问题 New state of 'nil' is invalid

...教程输入命令进入虚拟机时一直报这个“New state of 'nil' is invalid”。尝试卸载Docker Desktop后重启电脑重新安装还是这样。这种情况应该怎么办呢？