找到约 15 条结果

问题 差异基因用p-vaule筛选可以吗?

...and true positives. Moreover, when the number of DEGs obtained is low, it makes sense to look at other methods of modeling gene count data or reducing the FDR cutoff, etc. RNA-Seq being an exploratory technique for hypothesis generation, must be accompanied by multiple testing correction to avoid ...

文章 biom格式的文件如何转化

...header-key taxonomys less feature_table_tax.tsv # Constructed from biom file #OTU ID FT-1 CKR-4 CK0-1 CKR-5 FTR-4 CK0-5 CKR-1 FTR-5 FT-2 FT-3 CKR-3 CK0-4 CK0-3 FTR-3 FTR-2 FTR-1 FT-5 CK0-2 FT-4 CKR-2 taxonomy 6f8a1344c343b000bbe6c0946ef7af3f 3.0...

文章 染色体挂载:LACHESIS 安装

...sourceforge.net/projects/boost/files/boost/1.57.0/boost_1_57_0.tar.gz --no-check-certificate ./bootstrap.sh --prefix=/share/work/biosoft/boost/boost.1.57 --with-libraries=all ./b2 install ./bjam install

文章 shuf 随机抽取txt文件的行到新文件 linux命令

...ne  -n, --head-count=COUNT    output at most COUNT lines  -o, --output=FILE         write result to FILE instead of standard output      --random-source=FILE  get random bytes from FILE  -r, --repeat              output lines can be repeated  -z, --zero-terminated     line d...

问题 老师您好,我在运行megahit对200多个环境微生物宏基因组数据进行组装时,megahit报错Exit code -9

...536 reads to search for mercy k-mers' 2025-05-26 08:27:54 - Error occurs, please refer to /data2/temp/megahit/log for detail 2025-05-26 08:27:54 - Command: /home/anaconda3/envs/megahit/bin/megahit_core seq2sdbg --host_mem 973841920819 --mem_flag 1 --output_prefix /data2/temp/megahit/tmp/k21/21 --n...

文章 bam文件转换fastq

...bamToFastq)Version: v2.25.0Summary: Convert BAM alignments to FASTQ files.Usage:   bamToFastq [OPTIONS] -i <BAM> -fq <FQ>Options:        -fq2    FASTQ for second end.  Used if BAM contains paired-end data.                BAM should b...

问题 我在做T2T,docker下载T2T镜像错误

... koq0g34v.mirror.aliyuncs.com: StatusCode: 403, This request is forbidden. Please proceed to https...

文章 空间转录组数据读取

...置的函数直接读取了,代码如下 obj = Load10X_Spatial(data.dir = your_data_dir,filename = "filtered_feature_bc_matrix.h5",assay = assay) 这个datadir就是spaceranger跑出来的目录里有个子目录叫out,指定一下就行,后面的操作就和单细胞转录组差不多了,这个...

文章 pennCNV软件linux系统安装

...ithub.com/WGLab/PennCNV/archive/v1.0.4.tar.gztar xvfz v1.0.4.tar.gzcd kext/make 但是会报错: gcc `perl -MExtUtils::Embed -e ccopts` -fPIC -c -o khmm_wrap.o khmm_wrap.c khmm_wrap.c: In function ‘SWIG_AsCharPtrAndSize’: khmm_wrap.c:1576: error: ‘bool’ undeclared (first use in thi...

问题 putty 跑一半显示磁盘不够用

...d.txt|while read ind;do /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl --gff3 ./${ind}.longest_isoform.gff3 --fasta ./${ind}.fa.gz --seqType CDS >./${ind}.cds.fa /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl --gff3 ./${ind}.longest_isoform.gff3 --f...

问题 请问怎么从镜像里拷贝pl脚本2.0

 /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl 请问老师,这两个脚本怎么从镜像里下载呢。——————(以下是问题背景)——————课题组服务器不让安docker...

问题 WGCNA分析中,导入txt的性状数据时(数据为12行样本,10列性状数据),报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec, : line 1 didot have 12 elements。但是数据如果是12行样本,8列性状,不会报错

...Data = read.table("liunatrait1.txt",row.names=1,header=T,comment.char = "",check.names=F)但是报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  :    line 1 did not have 12 elements。但是数据如果是12行样本,8列性状,就不会报错。谢谢

文章 BUSCO评估基因组组装和完整性详解

...prok# or ignoring eukaryotes to save runtime, if compatible with your experimental goal.busco -m MODE -i INPUT -o OUTPUT --auto-lineage-euk# or ignoring non-eukaryotes to save runtime, if compatible with your experimental goal.  -i:基因组组装文件; -o:输...

文章 linux系统当中源码安装R,无root权限安装

...本及以上cd zlib-1.2.11 ./configure --prefix =/share/work/biosoft/zlib make make install2.安装bzipcd bzip2-1.0.6 make -f Makefile-libbz2_so 修改Makefile 中的PREFIX=/share/work/biosoft/bzip2 make && make install3.安装liblzma5.0.3版本及以上cd xz-5.2.3 ./configure -prefix=/share...

文章 racon 安装报错:

] Linking CXX executable bin/racon /share/work/biosoft/zlib/lib: file not recognized: Is a directory git clone https://github.com/lbcb-sci/racon && cd racon && mkdir build && cd build cmake -DCMAKE_BUILD_TYPE=Release .. && make cmake -DCMAKE_BUILD_TYPE=Releas...