...ge.vcf.gz
-c, --stdout write on standard output, keep original files unchanged
-f, --force overwrite files without asking
-@, --threads INT number of compression threads to use [1]
2. 对生成的vcf.gz进行index：
bcftools index [options] <in.bcf>|<i...
...d store response at var $resp
my $resp = $ua->get($url); # check response
if ($resp->is_success) {
# get response content(html source code)
my $content = $resp->decoded_content;
# use Regex get page title from $content
if ( ...
...and true positives.
Moreover, when the number of DEGs obtained is low, it makes sense to look at other methods of modeling gene count data or reducing the FDR cutoff, etc.
RNA-Seq being an exploratory technique for hypothesis generation, must be accompanied by multiple testing correction to avoid ...
...ne -n, --head-count=COUNT output at most COUNT lines -o, --output=FILE write result to FILE instead of standard output --random-source=FILE get random bytes from FILE -r, --repeat output lines can be repeated -z, --zero-terminated line d...
...prok# or ignoring eukaryotes to save runtime, if compatible with your experimental goal.busco -m MODE -i INPUT -o OUTPUT --auto-lineage-euk# or ignoring non-eukaryotes to save runtime, if compatible with your experimental goal.
-i：基因组组装文件;
-o：输...
...bamToFastq)Version: v2.25.0Summary: Convert BAM alignments to FASTQ files.Usage: bamToFastq [OPTIONS] -i <BAM> -fq <FQ>Options: -fq2 FASTQ for second end. Used if BAM contains paired-end data. BAM should b...