2025-12-26 17:43:56 [WARNING] line[285799] Discard this item[transcript] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285800] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285801] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285802] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285806] Discard this item[transcript] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285807] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285808] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285809] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285810] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285815] Discard this item[transcript] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285816] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285817] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285818] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285819] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285820] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285821] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285826] Discard this item[transcript] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285827] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285828] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285829] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285830] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285835] Discard this item[transcript] due to the parent detection failed, please check GTF/GFF
2025-12-26 17:43:56 [WARNING] line[285836] Discard this item[exon] due to the parent detection failed, please check GTF/GFF
运行anno日志
2025-12-26 18:25:05 [INFO ] Complete image quality control.
2025-12-26 18:25:06 [INFO ] Complete ipr file object serialization.
2025-12-26 18:26:07 [INFO ] Complete source image data compression.
2025-12-26 18:26:07 [INFO ] Delete intermediate files.
2025-12-26 18:26:27 [INFO ] Verify image data /work/mySTOmic/01.saw/out/BS_1/STEREO_ANALYSIS_WORKFLOW_PROCESSING/IMAGE/A04244C4_SC_20251226_182237_4.2.0.tar.gz file for registration.
2025-12-26 18:26:27 [INFO ] Image verification successful.
2025-12-26 18:26:27 [INFO ] Pipeline need to handle these subtask: ['stitch', 'register', 'tissueseg', 'cellseg'].
2025-12-26 18:26:27 [INFO ] >> Next do the Stitch task.
2025-12-26 18:26:27 [INFO ] Use the ScopeStitch stitching algorithm to calculate the stitching coordinates for stitching.
2025-12-26 18:26:31 [INFO ] Large image quality control, skip stitching module.
2025-12-26 18:27:43 [INFO ] >> Next do the Registration task.
2025-12-26 18:27:43 [INFO ] Get registration parameters.
2025-12-26 18:27:43 [INFO ] Successfully obtained the pre-registered track line template.
2025-12-26 18:27:44 [runtime] (rule_register) (error:register) 20406 2025-12-26 18:27:43 [ERROR ] SAW-A04002 PARSE ERROR: -v/--vis /work/mySTOmic/01.saw/out/BS_1/STEREO_ANALYSIS_WORKFLOW_PROCESSING/ANNOTATION/A04244C4.raw.gef is empty.
运行register日志，报错A04244C4.raw.gef is empty
经过排查怀疑是ref文件的问题
想问一下如何构建符合华大saw的参考基因组中的gtf注释文件
