在callsnp过程中形成db文件时出错,但是可以形成db文件夹,我继续往下做,gvcf转化成vcf得到的结果文件没有SNP行,只有上边的注释行和表头

err.79151

15:54:41.391 WARN  GenomicsDBImport - GenomicsDBImport cannot use multiple VCF reader threads for initialization when the number of intervals is greater than 1. Falling back to serial VCF reader initialization.

15:54:41.529 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.529 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.529 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.529 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.529 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.558 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.559 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.559 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.559 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.559 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.567 WARN  IntelInflater - Zero Bytes Written : 0

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15:54:41.585 WARN  IntelInflater - Zero Bytes Written : 0

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15:54:41.587 WARN  IntelInflater - Zero Bytes Written : 0

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15:54:41.607 WARN  IntelInflater - Zero Bytes Written : 0

15:54:41.617 INFO  GenomicsDBImport - Importing batch 1 with 5 samples

15:54:41.617 INFO  GenomicsDBImport - Importing batch 1 with 5 samples

15:54:41.618 INFO  GenomicsDBImport - Importing batch 1 with 5 samples

15:54:41.618 INFO  GenomicsDBImport - Importing batch 1 with 5 samples

15:54:41.618 INFO  GenomicsDBImport - Importing batch 1 with 5 samples


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1 个回答

马杰宇

以下是我的命令:/public/software/singularity/bin/singularity exec /public/home/perl5/gwas-sif/reseq.sif \

bash -c "gatk  --java-options "-Xmx20g" GenomicsDBImport  \
  -L chr.list --tmp-dir $tmpdir  -R $REF --batch-size 5 \
  --reader-threads 5 --max-num-intervals-to-import-in-parallel 5 \
  --genomicsdb-workspace-path db --sample-name-map cohort.sample_map"
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