我在运行到###alpha和beta多样性分析的时候
qiime diversity core-metrics-phylogenetic \
--i-phylogeny $workdir/3.asv_taxonomy/rooted-tree.qza \
--i-table $workdir/3.asv_taxonomy/feature-table-final.qza \
--p-sampling-depth 16900 \
--m-metadata-file $fastmap \
--output-dir core-metrics-results
运行过后出现下面的报错，请问各位大佬/老师该如何解决呢？
/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/sklearn/metrics/pairwise.py:1776: DataConversionWarning: Data was converted to boolean for metric jaccard
warnings.warn(msg, DataConversionWarning)
/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:143: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.05473873232362785 and the largest is 2.538043724148074.
warn(
Running external command line application. This may print messages to stdout and/or stderr.
The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
Command:
faithpd -i /work/tmp/qiime2/root/data/c800d091-b537-4e38-80d1-b7434419e2e0/data/feature-table.biom -t /work/tmp/qiime2/root/data/6ae5197f-066d-43fb-aa45-bf6b1f3a0f87/data/tree.nwk -o /work/tmp/q2-AlphaDiversityFormat-iimnpxr5
Running external command line application. This may print messages to stdout and/or stderr.
The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
Command:
ssu -i /work/tmp/qiime2/root/data/c800d091-b537-4e38-80d1-b7434419e2e0/data/feature-table.biom -t /work/tmp/qiime2/root/data/6ae5197f-066d-43fb-aa45-bf6b1f3a0f87/data/tree.nwk -m unweighted -o /work/tmp/q2-LSMatFormat-24m6d2q2
Current file: /opt/conda/conda-bld/unifrac-binaries_1659558422263/work/src/unifrac_cmp.cpp
function: _ZN6su_acc21UnifracUnweightedTaskIdE4_runEjPKd
line: 558
This file was compiled: -ta=tesla:cc35,cc50,cc60,cc60,cc70,cc75,cc80,cc80
Traceback (most recent call last):
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2cli/commands.py", line 339, in __call__
results = action(**arguments)
File "<decorator-gen-289>", line 2, in core_metrics_phylogenetic
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 234, in bound_callable
outputs = self._callable_executor_(scope, callable_args,
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 475, in _callable_executor_
outputs = self._callable(scope.ctx, **view_args)
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity/_core_metrics.py", line 66, in core_metrics_phylogenetic
dms += unweighted_unifrac(table=cr.rarefied_table, phylogeny=phylogeny,
File "<decorator-gen-529>", line 2, in unweighted_unifrac
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 234, in bound_callable
outputs = self._callable_executor_(scope, callable_args,
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/qiime2/sdk/action.py", line 381, in _callable_executor_
output_views = self._callable(**view_args)
File "<decorator-gen-259>", line 2, in unweighted_unifrac
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity_lib/_util.py", line 69, in _disallow_empty_tables
return wrapped_function(*args, **kwargs)
File "<decorator-gen-258>", line 2, in unweighted_unifrac
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity_lib/_util.py", line 112, in _validate_requested_cpus
return wrapped_function(*bound_arguments.args, **bound_arguments.kwargs)
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity_lib/beta.py", line 221, in unweighted_unifrac
_omp_cmd_wrapper(threads, cmd)
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity_lib/_util.py", line 128, in _omp_cmd_wrapper
return _run_external_cmd(cmd, verbose=verbose, env=env)
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/site-packages/q2_diversity_lib/_util.py", line 122, in _run_external_cmd
return subprocess.run(cmd, check=True, env=env)
File "/biosoft/miniconda/envs/ampliseq-q2/lib/python3.8/subprocess.py", line 516, in run
raise CalledProcessError(retcode, process.args,
subprocess.CalledProcessError: Command '['ssu', '-i', '/work/tmp/qiime2/root/data/c800d091-b537-4e38-80d1-b7434419e2e0/data/feature-table.biom', '-t', '/work/tmp/qiime2/root/data/6ae5197f-066d-43fb-aa45-bf6b1f3a0f87/data/tree.nwk', '-m', 'unweighted', '-o', '/work/tmp/q2-LSMatFormat-24m6d2q2']' returned non-zero exit status 1.