动植物基因组组装课程,结构注释部分,运行braker.pl出错

脚本:03.Braker.sh  第78行:

braker.pl --species=$species \

   --genome=$contig \

   --softmasking \

   --bam=rnaseq.bam \

   --cores=$threads \

   --useexisting   \

   --gff3 --skip_fixing_broken_genes


系统提示:

Failed  to create new species with new_species.pl, check write permissions in /share/work/biosoft/augustus/latest/config//species directory!     Command was perl /share/work/biosoft/augustus/augustus-3.3.3/scripts/new_species.pl --species=Acer_truncatum --AUGUSTUS_CONFIG_PATH=/share/work/biosoft/augustus/latest/config/ 1> /dev/null 2>/home/shpc_100675/workspace/zxdjt/animal/annotation/03.Braker/braker/errors/new_species.stderr


问题:
1、/share/work/biosoft/augustus/latest/config//species :contig后面怎么有两个斜线?用cd命令,正常应该是/share/work/biosoft/augustus/latest/config/species


2、根据提示,查看了目录species的权限,发现是可读可写可执行属性(drwxrwxrwx 111 root root 2556 Aug 23  2022 species),但是在species内新建目录,提示如下。怎么解决不能写入文件的问题?

mkdir mine

mkdir: cannot create directory ‘mine’: Read-only file system

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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

刚刚测试了我们的镜像,没问题的,attachments-2023-02-YHDd9RDH63fc1daf78779.png

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  • cashing 提出于 2023-02-27 00:15

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