tassel 报错 SortGenotypeFilePlugin to correctly order the file.

ERROR net.maizegenetics.plugindef.AbstractPlugin - Problem loading file: /work/my_pop_cucu/00.filter/clean.vcf.gz. Error: BuilderFromVCF: Ordering incorrect. VCF file must be ordered by position. Please first use SortGenotypeFilePlugin to correctly order the file.

请先 登录 后评论

2 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

排下序就行,用排好序的文件再输入即可避免错误:

#利用tassel软件对文件进行排序
run_pipeline.pl -Xmx30G  -SortGenotypeFilePlugin -inputFile clean.vcf.gz \
    -outputFile clean.sorted.vcf.gz -fileType VCF

#vcf文件格式转换成Phylip格式,用于后续构建进化树
run_pipeline.pl  -Xmx5G -importGuess  $workdir/00.filter/clean.sorted.vcf.gz  \
    -ExportPlugin -saveAs supergene.phy -format Phylip_Inter
请先 登录 后评论
Marina

你好,在用水稻3k数据库的ped去跑plink的时候,遇到了这样的报错:

[pool-1-thread-1] ERROR net.maizegenetics.plugindef.AbstractPlugin - BuilderFromPLINK: build: problem processing: / by zero

请先 登录 后评论