找到约 15 条结果

问题 docker vitualbox安装报错:Unable to start the VM: C:\Program Files\Oracle\VirtualBox\VBoxManage.exe startvm default

docker vitualbox安装时报错:Unable to start the VM: C:\Program Files\Oracle\VirtualBox\VBoxManage.exe startvm default --type headless failed: VBoxManage.exe: error: The virtual machine 'default' has terminated unexpectedly during startup with exit code -1073741819 (0xc0000005) VBoxManage.exe: ...

文章 samtools使用

...t alignment file split splits a file by read group quickcheck quickly check if SAM/BAM/CRAM file appears intact fastq converts a BAM to a FASTQ fasta converts a BAM to a FASTA -- Statistics bedcov read depth per BED region depth...

问题 遗传图谱构建

...件夹为空,部分错误代码如下: IOError: [Errno 2] No such file or directory: 'pop.bin.12' warning, cmd: snpbinner visualize --out bin.visualize.12 --crosspoints pop.crosspoints.12  --snps pop.forbin.12 --bins  pop.bin.12 failed with ret: 256, going to retry. warning, cmd: snpbinne...

问题 tassel新报错,求老师们看一下[Thread-8] ERROR net.maizegenetics.plugindef.AbstractPlugin - Index 0 out of bounds for length 0

...generated? (BLUE = best linear unbiased estimate) (Default: false) -saveToFile <true | false> : Should the results be saved to a file rather than stored in memory? It true, the results will be written to a file as each SNP is analyzed in order to reduce memory requirementsand the results will...

文章 bedtools安装报错cc1plus: error: unrecognized command line option "-std=c++11"

... 20120313 (Red Hat 4.4.7-17) (GCC), CURRENT_VERSION  =Updating version file. * Creating BamTools API- Building in src/utils/FileRecordTools  * compiling FileRecordMgr.cppcc1plus: error: unrecognized command line option "-std=c++11"make[1]: *** [../../../obj//FileRecordMgr.o] Error 1make: *** [...

问题 在建立索引时出错,输出文件都是0KB

...and line syntax is changing. ********** ********** For more information, please see: **********  https://github.com/broadinstitute/picard/wiki/Command-Line-Syntax-Transition-For-Users-(Pre-Transition) ********** ********** The command line looks like this in the new syntax: ********** *****...

问题 基因组注释提取,gff3_file_to_proteins.pl 序列出错,跟官网给的序列不一致

  /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl  --gff3  $species.longest_isoform.gff3  --fasta  $genome --seqType prot >$species.pep.fa 这一部分的分析,提取出来的蛋白序列与官方发布的蛋白序列有出入,而且U等氨基酸字母会直接被...

问题 老师好,我在利用在SNP设计dcasp标记时得到的primers.result.xls文件为空。

这是我的报错: rm: cannot remove ‘/work/out/tmp_in/*’: No such file or directory Options done Output selected CAPS file name is:  CAPS_output/selected_CAPS_primers.txt ids is  [] SNP_A, SNP_B  G A CAPS found with enzyme  BstNI,PspGI,19 One nt can be changed to fit enzyme NlaIII...

问题 mysql镜像启动问题

...-24 13:44:46+00:00 [ERROR] [Entrypoint]: mysqld failed while attempting to check config command was: mysqld --verbose --help --log-bin-index=/tmp/tmp.TNVtIp74qF mysqld: Can't read dir of '/etc/mysql/conf.d/' (Errcode: 2 - No such file or directory) mysqld: [ERROR] Fatal error in defaults handling...

文章 GATK|Timeout of 30000 milliseconds was reached while trying to acquire a lock on file raw.vcf.tmp.idx.

解决办法:https://www.omicsclass.com/article/2194 这里还有一个需要做的步骤,就是先将raw.vcf.tmp.idx文件找到并删除,一般情况下这个文件是空的。 然后再在命令行中添加-disable_auto_index_creation_and_locking_when_reading_rods 这个参数。并运...

文章 qiime2 couldn't connect to display "localhost:12.0"

Traceback (most recent call last): File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__ results = action(**arguments) File "<decorator-gen-545>", line 2, in summarize File "/share/work/biosoft/conda/Mini...

问题 python rpy2 报错

... robjError: package or namespace load failed for ‘methods’ in dyn.load(file, DLLpath = DLLpath, ...): unable to load shared object '/share/work/biosoft/R/R-v4.0.3/lib64/R/library/methods/libs/methods.so':  /share/work/biosoft/R/R-v4.0.3/lib64/R/library/methods/libs/methods.so: undefined symbol...

问题 使用tassel对vcf文件进行 -SortGenotypeFilePlugin 基因型排序时自动锅炉掉了我的两条contig,这个问题怎么解决呀?

我要做Gwas分析,需要生成hapmap文件,但是我直接用vcf转hapmap会报错Please first use SortGenotypeFilePlugin to correctly order the file.所以我就用tassel去排序然后可以正常生成hapmap文件了,但是我的两条比较小的contig不见了

问题 非模式物种建立注释库问题

...umn types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") :   cannot open file 'NA/pathway2name.tsv': No such file or directory Execution halted 已经...

文章 biolinux安装matplotlib时报错

...fo for package tornado     Traceback (most recent call last):       File "<string>", line 17, in <module>       File "/tmp/pip_build_root/tornado/setup.py", line 146, in <module>         raise ImportError("Tornado requires an up-to-date SSL module. This means "   ...