...CAF（devCAFs）
Supplementary Table 5 Genes used to define CAFs in the scRNA-seq dataset andtheir reference (Fig. 1h).Cell typeGene nameReferenceFibroblasts/CAFsBGNOur spatial transcriptomics data (enriched in omental metastasis), verified high expression specifically in fibroblasts in the ...
[root@iZ2e]# ./gemma-0.98.5-linux-static-AMD64 -bfile c -k output/result1.sXX.txt -lmm 1 -p p.txt -c c.txt
GEMMA 0.98.5 (2021-08-25) by Xiang Zhou, Pjotr Prins and team (C) 2012-2021
ERROR: Enforce failed for file_kin output/result1.sXX.txt: open file in src/param.cpp at line 934 in CheckParam
...，均报下列错：
ERRO[0000] invalid internal status, try resetting the pause process with "/usr/bin/podman system migrate": could not find any running process: no such process
以及前期在docker中进行进化树构建时，进程经常自动中止（docker 创建时指定了最大使用...
...形界面，否则会报如下的错误_tkinter.TclError: no display name and no $DISPLAY environment variable
一个可能的解决方案是在matplotlib中使用"Agg"后端，它可以在没有显示设备的情况下生成图形
import matplotlibmatplotlib.use('Agg')
当然，很多时候...
...ed early on line 266653. Expected 9 fields but found 1. Consider fill=TRUE and comment.char=. First discarded non-empty line: <<##sequence-region chr2 1 242193529>>
...ed nucleotide alignments guided by amino acid alignments are more reliable and accurate than direct nucleotide alignments)。该方法尤其对于序列差异较大的基因对，比对更准确，可以避免由于比对问题导致KaKs_Calculator计算不出结果。
下载安装和数据准备
...
There was a problem importing /work/my_amplicon/A/manifest.ckd.txt:
/work/my_amplicon/A/manifest.ckd.txt is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "forward-absolute-filepath" could not be found ($datadir/SRR7441413_1.fastq) for sample "SRR7441413".
... correction by racon with tgs reads.
- TGS reads type is ont . MINIMAP2_PARAM is -x ava-ont MIN_IDY is 0.3 . MIN_MATCH is 2000 .
INFO : Checking basic args & env end.
INFO : Step 1 , run TGSSeqSplit to split scaffolds into scaftigs(contigs).
...