找到约 15 条结果

文章 Evolview:设置标签的装饰

...05)100:0.1)90:0.43;”。 [2].   点击Annotation upload → upload data for leaf label decorations,在data文本框输入“A triangle,red”并提交。 [3].   结果展示如下 命令格式说明: 设置语句至少有2列:location shape,color[:stroke_color],由制表符分...

问题 老师我想问一下,MENA在analysis the network 报错的问题

...lization(cor.matrix,stresscent)centralization(cor.matrix,evcent)sna: Tools for Social Network AnalysisVersion 2.3-2 created on 2014-01-13.copyright (c) 2005, Carter T. Butts, University of California-IrvineFor citation information, type citation("sna").Type help(package="sna") to get started.Error i...

文章 awk正则表达式使用外部变量

...hell变量,先在变量外加双引号,再加单引号即可,如: for i in read write print; do cat file.txt | awk '{ if ($7~/'"$i"'/) {print $0} }'| wc -l ; done 在写shell脚本时,经常会使用到awk程序。但是有些复杂的逻辑,可能需要在awk中使用在shell中...

文章 Logistic回归模型介绍及使用(附脚本)

...iate ...], --variate variate [variate ...] variate for cox analysis[required] -l pvalue, --pvalue pvalue pvalue cutoff to choose sig gene[default 0.05] --log2 whether do log2 transfrom for expression data[opti...

问题 gVCF文件转换成VCF格式时,已经在后台运行了很久了,突然程序关闭了,报错信息显示:

...llegalStateException: No likelihood sum exceeded zero -- method was called for variant data with no variant information.         at org.broadinstitute.hellbender.tools.walkers.genotyper.AlleleSubsettingUtils.calculateMostLikelyAlleles(AlleleSubsettingUtils.java:31>         at org.broadi...

问题 docker运行qiime1时按照教程进行到章节2课时6时,即聚类生成OTU-denovo时老是报错,查看类似问题后,升级了运行内存为70g,同时将教程内的30多个样本减成2个样本,FASTQ格式还有qimme格式检查都正确,依旧报错,报错内容如下:

...n2.7/site-packages/matplotlib/font_manager.py:273: UserWarning: Matplotlib is building the font cache using fc-list. This may take a moment.   warnings.warn('Matplotlib is building the font cache using fc-list. This may take a moment.') Traceback (most recent call last):   File "/biosoft/minico...

问题 重复序列注释问题

...组重复序列注释时,出现如下错误该如何解决?  W tensorflow/stream_executor/platform/default/dso_loader.cc:64] Could not load dynamic library 'libcuda.so.1'; dlerror: libcuda.so.1: cannot open shared object file: No such file or directory; LD_LIBRARY_PATH: /share/work/biosoft/mpi...

文章 bioperl批量计算蛋白质分子量等电点pI

...蛋白序列就可以:蛋白分子量分析:ExPASy (http://web.expasy.org/protparam/)来计算: 但是这个网站,只能一条一条的提交序列然后计算,如果后很多条序列要提交,其不支持批量提交,所有功能有限,这里我写了个perl脚本,利用b...

文章 一行代码检查GWAS分析中表型数据的错误

... 12 11 7 运行该代码 awk 'NR == 1 {print "Line", NR, $0; next} {for (i = 2; i <= NF; i++) if ($i !~ /^[0-9.]+$/ && $i != "NA") {print "Line", NR, $0; break}}' trait.txt 得到结果如下,会展示出表头及除了数字和NA之外的所有字符以及所在的行数,...

问题 转录组上游分析之定量比对Hisat2出现内部异常错误

...singles> file cannot also be specified.  Please run HISAT2 separately for mates and singles. Error: Encountered internal HISAT2 exception (#1) Command: /home/vip6/miniconda3/envs/rna/bin/hisat2-align-s --wrapper basic-0 -p 3 -S /home/vip6/project/human-64-Asthma-Trans/Mapping/Hisat//SRR103950...

文章 进化树+基因表达热图二合一靓图绘制教程

...nnotation upload”下的第11个选项绘制热图,点击“upload data for heatmap(help)”可进入绘制热图说明文档,参数设置可以参考左上角heatmaps案例,在弹出页面上提交拟南芥基因表达量,其它得到最终进化树(3)在Export中导出结果,常用p...

问题 docker运行qiime1时按照教程进行到章节2课时6时,即聚类生成OTU-denovo时老是报错,查看类似问题后,升级了运行内存为70g,同时将教程内的30多个样本减成2个样本,FASTQ格式还有qimme格式检查都正确,依旧报错,报错内容如下:Segmentation fault

...n2.7/site-packages/matplotlib/font_manager.py:273: UserWarning: Matplotlib is building the font cache using fc-list. This may take a moment.   warnings.warn('Matplotlib is building the font cache using fc-list. This may take a moment.') Traceback (most recent call last):   File "/biosoft/minico...

文章 MUSCLE软件介绍

...log: MUSCLE v3.8.31 by Robert C. Edgar http://www.drive5.com/muscle This software is donated to the public domain. Please cite: Edgar, R.C. Nucleic Acids Res 32(5), 1792-97. 1 2 seqs, max length 19, avg  length 15 00:00:00    11 MB(-1%)  Iter   1  100.00%  K-mer dist pass 1 00:00:00 ...

问题 请问为什么GWAS用tassel mlm模型画的manhattan图什么都没有呢 也没有报错 能生成一个什么都没有的图片 只有坐标和题

...ains_per_panicle -n grains_per_panicle_manhattan -c 1.3e-6 --> Q&A for bioinformatics, please visit the website: www.omicsclass.com --> Recommended to learn R language: --> https://study.163.com/course/introduction/1209073807.htm?share=1&shareId=1030291076 Loading required packa...

文章 如何计算蛋白序列的长度、分子量、等电点等信息

...::SeqIO;use Bio::Seq;use Bio::Tools::SeqStats;use Bio::Tools::pICalculator;use Data::Dumper;#读入序列my $in = Bio::SeqIO->new(        -file   => "$ARGV[0]",        -format => 'Fasta');open OUT,">$ARGV[1]" or die "$!";print OUT "#ID\tlength\tMV(Da)\t...