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参考文献:
[1] Edgar, R.C. Updating the 97% identity threshold for 16S ribosomal RNA OTUs. (2017). doi:https://doi.org/10.1101/192211
[2] Callahan,B. J., P. J. McMurdie, M. J. Rosen, A. W. Han, A. J. A. Johnson and S. P.Holmes (2016). "DADA2: High-resolution sample inference from Ill...
...这些gvcf文件,但是提示我错误:A USER ERROR has occurred: The list of input alleles must contain <NON_REF> as an allele but that is not the case at position 763769; please use the Haplotype Caller with gVCF output to generate appropriate records。由于缺乏bam文件我只能尝...
...,显示warning skip Indel site, there are total skip Indel sites number is : 105。
因为我的vcf文件中同时包括了snp和indel变异信息,有些indel位点与表型是显著关联的,我想保留indel位点,并在热图中与snp数据同时可视化,类似下图这样。请问应...
在进行批量indel标记设计的时候,出现如下错误,[ERRO] illegal DNA/RNA/Protein sequence: 248, you may switch on -d/--degenerate or -r/--use-regexp
原因好像是因为基因组序列里有简并碱基,所以怎么处理,使能够正常设计引物
perl script/mRNAid_to_geneid.pl Arabidopsis_thaliana.TAIR10.41.gff3 mRNA2geneID.txt
在执行这个指令时,出现:Can't open perl script "script/mRNAid_to_geneid.pl": No such file or directory 错误,是perl脚本没有封装在容器中吗?
...tdir.strip()+'/outfile','/'+args.outdir.strip()+'/infile')
FileNotFoundError: [Errno 2] No such file or directory: '//work/gene_family_demo/03.gene_tree_analysis/outfile' -> '//work/gene_family_demo/03.gene_tree_analysis/infile'
请问如何解决?在使用本地mega建nj树几乎不可能...
> library(qqman)
> results_log = read.table("logistic_results.assoc.logistic", head=TRUE)
Error in file(file, "rt") : cannot open the connection
In addition: Warning message:
In file(file, "rt") :
cannot open file 'logistic_results.assoc.logistic': No such file or directory
...HUB_PAT. Use `gitcreds::gitcreds_set()` and unset GITHUB_PAT in .Renviron (or elsewhere) if you want to use the more secure git credential store instead.
Error: Failed to install 'unknown package' from GitHub:
HTTP error 401.
Bad credentials
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...l, default: 1]
-X x.lab, --x.lab x.lab
the label for x axis [optional, default: log2FC]
-Y y.lab, --y.lab y.lab
the label for y axis [optional, default: -log10(FDR)]
-T title, --title title
the label for main title [option...