我在运行Launch_PASA_pipeline.pl -f FL_accs.txt -c alignAssembly.config -C -r -R -g genome.softmasked.fa -T -t all_transcripts.fasta.clean -u all_transcripts.fasta --CPU $threads --ALIGNERS blat,minimap2 --TRANSDECODER --TDN tdn.accs --MAX_INTRON_LENGTH 1000000
这步时报错，
minimap2 TRINITY_GG_8151_c3_g1_i1 122192 1151017 chr9_RagTag 1 + ? OK 30632781-30633198 99.8 100 41716.4 orient(a+/s?) align: 30632781(1)-30633198(418)
minimap2 TRINITY_GG_17288_c9_g1_i1 248811 1151018 chr9_RagTag 1 - ? OK 30634002-30634247 100 100 24600 orient(a-/s?) align: 30634002(246)-30634247(1)
Completed.
* [Sun Oct 19 21:42:40 2025] Running CMD: /share/work/biosoft/PASApipeline/PASApipeline-v2.5.2/scripts/update_alignment_status.dbi -M '/work/0509t2t/18.PASA/pasa.sqlite' < alignment.validations.output > pasa_run.log.dir/alignment.validation_loading.output
sh: alignment.validations.output: No such file or directory
Error, cmd: /share/work/biosoft/PASApipeline/PASApipeline-v2.5.2/scripts/update_alignment_status.dbi -M '/work/0509t2t/18.PASA/pasa.sqlite' < alignment.validations.output > pasa_run.log.dir/alignment.validation_loading.output died with ret 256 No such file or directory at /share/work/biosoft/PASApipeline/PASApipeline-v2.5.2/PerlLib/Pipeliner.pm line 187.
Pipeliner::run(Pipeliner=HASH(0x7fc3a0)) called at /share/work/biosoft/PASApipeline/latest/Launch_PASA_pipeline.pl line 1047
请问是什么原因？而且我发现好多所有这种临时文件都是空的
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.2
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.1
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.6
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.5
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.4
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.3
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.9
-rw-r--r--. 1 root root 0 Oct 19 13:46 tmp-2762815-68285-out.tmp.8
