文章 宏基因组的基因组binning-BASALT 介绍和用法

... References： Qiu, Z., Yuan, L., Lian, CA. et al. BASALT refines binning from metagenomic data and increases resolution of genome-resolved metagenomic analysis. Nat Commun 15, 2179 (2024). https://doi.org/10.1038/s41467-024-46539-7https://github.com/EMBL-PKU/BASALThttps://mp.weixin.qq.com/s/Tlxai...

话题 Too many levels of symbolic links

问题 泛基因家族分析-SV和基因表达量

fpkm_SV_to_draw.2.txt文件中，为啥结果是这样，基因ID对应同一个基因组，这不对吧。

问题 geneid_to_mRNAid.pl

输入文件： 报错：

问题 用deseq_analysis.r分析差异表达分析时报如下的错误

[1] "abstract_factors is start" Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  :   line 2 did not have 3 elements Calls: abstract_factors -> read.table -> scan Execution halted

问题 root@jieyuma-virtual-machine:/home/jieyuma/Desktop# docker search omicsclass Error response from daemon: Get "https://index.docker.io/v1/search?q=omicsclass&n=25": dial tcp: lookup index.docker.io on 127.0.0.53:53: server misbehaving

在两个不同电脑同时出现了这个问题，一直解决不了，麻烦老师了

问题 16S课程 02.feature_table #16S 分析 deblur

...a \     --p-trim-length 400 \     --p-sample-stats \     --p-jobs-to-start 10 \     --p-min-reads 2 \     --o-representative-sequences deblur-repset-seqs.qza  \     --o-table deblur-table.qza \     --o-stats deblur-stats.qza There was a problem loading '../01.import_data/dem...

文章 单细胞转录组数据挖掘流程记录-肝细胞癌（HCC）(GSE149614)

...4.afterQC.rds \ -p GSE149614 --resolution 0.5 -d 30 \ -o 02.cluster --integrate.method harmony --batch.id sample \ --vars.to.regress nUMI percent_mito --high.variable.genes 2000 结果展示： 单细胞转录组分析课程推荐：https://bdtcd.xetslk.com/s/4i88K6

问题 下载甲基化数据时找不到样本类型，

....type) :   Primary solid Tumor was not found. Please select a difinition from the table above 

问题 tassel 报错 SortGenotypeFilePlugin to correctly order the file.

ERROR net.maizegenetics.plugindef.AbstractPlugin - Problem loading file: /work/my_pop_cucu/00.filter/clean.vcf.gz. Error: BuilderFromVCF: Ordering incorrect. VCF file must be ordered by position. Please first use SortGenotypeFilePlugin to correctly order the file.

问题 mRNAid_to_geneid.pl出现错误，结果文件只有一行

老师，问答页面不能显示图片，我现在有个问题:gff文件是这样的：，运行命令perl script/mRNAid_to_geneid.pl Arabidopsis_thaliana.TAIR10.41.gff3 mRNA2geneID.txt得到的文件是这样的， ，请问怎么解决啊？

问题 mRNA_to_geneid.pl从哪里下载的

问题 R安装包报错ERROR: failed to lock directory

ERROR: failed to lock directory ‘/home/huangls/biosoft/miniconda3/envs/rna-seq/lib/R/library’ for modifying Try removing ‘/home/huangls/biosoft/miniconda3/envs/rna-seq/lib/R/library/00LOCK-stringi’

话题 gff3_file_to_proteins.pl

问题 老师您好！我在使用ParaAT是报错，提示Generating homologous group files for alignments: 0，请问这个如何解决？

...tched codons = FALSE Verbose output = FALSE Reading homologous groups from homo_pairs.txt1: 100 groups Reading nucleotide sequences from Input_cds.fa: 118339 nucleotide sequences Reading amino acid sequences from Input_pep.fa: 118339 amino acid sequences Generating homologous group files for...