找到约 15 条结果

文章 Infercnv|单细胞转录组拷贝数分析

... -i rds_data, --rds_data rds_data input rds_data file path[required] --assay assay Name of assay to use, defaults to the active assay [default RNA] --downsample downsample subset cells numbers for analysis [default...

问题 麻烦老师问一下,在做重测序的时候这个地方老是出错是怎么回事,路径都仔细检查过了,谢谢老师。no such file

文章 相关性分析及散点图绘制-脚本使用

...nal,default 8] -o OUTDIR, --outdir OUTDIR output file directory[optional,default cwd] -p PREFIX, --prefix PREFIX out file name prefix[optional,default kegg] 根据自身需要调整必要的参数: -n 输入列名,指定进行相关性分析...

问题 请问为大神,这种情况该怎么处理?

...="C:/Users/dong/Desktop/2/GSE40807_RAW"setwd(workdir)dir.create("result") files = list.files(workdir, pattern = "GSM")filesRawData=read.maimages(files=files,source="agilent.median",green.only = T)show(RawData) EListRaw=RawDatadim(EListRaw)colnames(EListRaw) BgCorrect=backgroundCorrect(EListRaw,...

文章 Evolview:设置标签的装饰

...状的类型 选项功能triangle三角形circle圆形rect矩形star星形check勾形(√) 命令示例 [1].   三角形,填充色为红色,边框色为绿色(A   triangle,red:green) [2].   三角形,填充色为白色,边框色为红色(A   triangle,white:red) [3]...

文章 linux-列合并

...ello   WorldHello   WorldHello   WorldHello   World 命令为: paste file1 file2 > result 参数 -d 可指定连接符: paste -d '@' file1 file2 > result 结果为: Hello@WorldHello@WorldHello@WorldHello@World

问题 执行ALLHiC分组不成功

...tion -b sample.clean.bam -r draft.asm.fasta -e DPNII -k $k ## Extract CLM file and counts of restriction sites  #(HindIII: AAGCTT; MboI: GATC) allhic extract sample.clean.bam draft.asm.fasta --RE GATC ## 排序和方向优化 for K in `seq  1 $k`; do     allhic optimize sample.clean.coun...

文章 linux中gcc与pip安装

...只需两条命令: yum -y install gcc gcc-c++ autoconf pcre pcre-devel make automakeyum -y install wget httpd-tools vim 就把gcc当成c语言编译器, g++当成c++语言编译器用 注意安装需要root权限。 Pip安装 pip安装需下载一脚本,然后运行改脚本即可。...

问题 infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: -> readrds execution halted

infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: <anonymous> -> readrds execution halted

文章 rocks mysql数据库多实例数据库配置

...g mysql mysql cd /opt/rocks/mysql/scripts/./mysql_install_db --defaults-file=/share/work/mysql/3306/my.cnf --user=mysql --basedir=/opt/rocks/mysql --datadir=/share/work/mysql/3306/data #启动 /opt/rocks/mysql/bin/mysqld_safe --defaults-file=/share/work/mysql/3306/my.cnf --skip-grant-tables ...

问题 meme分析报错:Illegal character `U' in sequence LOC105388627. Change alphabet or fix data file.

请问老师,是根据最终得基因ID提取得蛋白质序列,为什么在做meme分析时,会出现这种错误?

文章 iTOL修改进化树的标签基因ID名称

...ata below (TAB,SPACE or COMMA).This separator must be used throughout this file (except in the SEPARATOR line, which uses space).SEPARATOR TAB#SEPARATOR SPACE#SEPARATOR COMMA#Internal tree nodes can be specified using IDs directly, or using the 'last common ancestor' method described in iTOL help pa...

问题 eggNOG 保留蛋白序列时提示同一 ID 被多个一级特征使用,而子特征的 Parent 属性没有得到相应更新,导致注释关系不明确的问题,该怎么解决

... -o ga_protein_coding.gff3 Indeed we changed the ID for this L1 feature to be unique but we do not change the Parent attribute of the child features to reflect this change. Why? because we do not know to which L1 the child feature was part-of because several Parent have similar ID. @ the feature...

文章 Linux中for循环使用

...------------for2-1.sh #!/bin/bash for i in `ls`; do echo $i is file name\! ; done   ----------------------------- for2-3.sh #!/bin/bash for i in f1 f2 f3 ; do echo $i is appoint ; done -----------------------------for2-4.sh #!/bin/bash list="rootfs usr data...

文章 基因组组装GC 含量与深度图:

...="character",required=T, default=NULL,help="input single Seurat obj in rds file [required]",metavar="filepath")parser$add_argument( "-d", "--depth.table", type="character",required=T, default=NULL,help="input single Seurat obj in rds file [required]",metavar="filepath")parser$add_argument( "--mingc"...