找到约 15 条结果

文章 KEGG GO 富集显著性图 名字太长,柱状图上文字解决

...创建数据框data <- data.frame(  Description = c(    "KEGG_STARCH_AND_SUCROSE_METABOLISM",    "KEGG_PENTOSE_AND_GLUCURONATE_INTERCONVERSIONS",     "KEGG_METABOLISM_OF_XENOBIOTICS_BY_CYTOCHROME_P450",    "KEGG_PORPHYRIN_AND_CHLOROPHYLL_METABOLISM",    "KEGG_NEUROACTIVE_LIGAND_RECEPT...

问题 OMIM数据库位置信息是hg19/GRCH37还是hg38/GRCH38为参考的?

What reference assembly does OMIM use for the genomic coordinates?

问题 老师请问这个没有共线性结果是哪里出问题了

...e-05 # MAX GAPS: 25 ############### Statistics ############### # Number of collinear genes: 0, Percentage: 0.00 # Number of all genes: 55 ########################################## ST_AT.collinearity (END)

文章 空间转录组数据读取

...ject_name = "SeuratProject", filter_matrix = TRUE) { # Function to read the count matrix and create Seurat object read_count_matrix = function(data_dir) { counts = Read10X(paste0(data_dir, "/filtered_feature_bc_matrix")) seurat_object = CreateSeuratObject(counts = counts, assay =...

问题 clustalw2多序列比对,序列输入不进去

...son (Fasta), GDE, Clustal, GCG/MSF,                  RSF.Enter the name of the sequence file :我输入了我的fasta格式的序列文件名,回车,出现:Sequence format is Pearson********************************************************************** CLUSTAL 2.1 Multiple Sequence Align...

文章 使用peer根据表达量数据推断隐藏因子

...藏因子,则与样本的数量有关,可以参考如下的标准: The number of PEER factors was selected as function of sample size (N): 15 factors for N < 15030 factors for 150 ≤ N < 25045 factors for 250 ≤ N < 35060 factors for N ≥ 350 根据文章的提示,使用的是...

文章 GATK外显子测序数据肿瘤体细胞突变数据分析

...rences_hg38_v0_Homo_sapiens_assembly38.dbsnp138.vcf \ --known-sites Mills_and_1000G_gold_standard.indels.hg38.vcf.gz -I N154.sort.dedup.bam -O N154.sort.dedup.bam.tablegatk BaseRecalibrator -R Homo_sapiens_assembly38.fasta \ --known-sites references_hg38_v0_Homo_sapiens_assembly38.dbsnp138.vc...

问题 docker vitualbox安装报错:Unable to start the VM: C:\Program Files\Oracle\VirtualBox\VBoxManage.exe startvm default

...he VM: C:\Program Files\Oracle\VirtualBox\VBoxManage.exe startvm default --type headless failed: VBoxManage.exe: error: The virtual machine 'default' has terminated unexpectedly during startup with exit code -1073741819 (0xc0000005) VBoxManage.exe: error: Details: code E_FAIL (0x80004005), component...

文章 PICRUSt2扩增子功能预测与差异比较分析

...PCAf分析pathway_pca(abundance = abundance_data, metadata = metadata, "type") 功能丰度差异比较分析 # Perform pathway DAA using ALDEx2 method# Please change column_to_rownames() to the feature column if you are not using example dataset# Please change group to "your_group_column...

问题 SNP过滤de问题

...   --recode               --stdout After filtering, kept 320 out of 320 Individuals       Outputting VCF file...       After filtering, kept 0 out of a possible 40089272 Sites       No data left for analysis!       Run Time = 2539.00 seconds 求助一下

文章 blast 中的e-value值如何理解?

...概念看起来就相对的比较模糊了,官方的定义是e-value:the number of hits you expect to see by chance/false positive . 对于定义里面的“by chance”,也就是随机情况下指的是什么意思不是很了解,随后查阅了一下文献,是这样解释的: by chan...

文章 qiime1 matplotlab绘图报错解决:_tkinter.TclError: no display name and no $DISPLAY environment variable

...4.Qiime_analysis/taxa_summary_plot/taxa_summary_plots/ --x_width 8 --chart_type bar --bar_width 0.5Command returned exit status: 1Stdout:StderrTraceback (most recent call last):  File "/share/work/biosoft/python/Python-v2.7.11/bin/plot_taxa_summary.py", line 278, in <module>    main()  Fil...

文章 【前沿研究】肺癌转移机制的新靶点-- lncRNA AC026904.1

...Long noncoding RNAs AC026904.1 is essential for TGF-β-induced migration and epithelial-mesenchymal transition through functioning as an enhancer of Slug in lung cancer cells”的文章,研究lncRNA AC026904.1在肺癌转移中的作用。发现AC026904.1在转录水平上调Slug表达,进...

文章 Linux命令处理文件合并

...e和awk处理: 命令如下: paste file1.txt file2.txt | awk 'BEGIN {OFS="\t"} {print $1, $2, $3, $4 + $11, $5 + $12, $6, $7}'  > result.txt 使用paste命令将两个文件合并,再使用awk命令计算第4列和第5列的和,并保持其他列不变,输出以tab键分隔: aw...

问题 单细胞测序数据差异基因KEGG富集分析

...rocyte.AvsB --pvalueCutoff 0.05 --ann.db org.Ss.eg.db --organism ssc  --idtype SYMBOL报错:nohup: ignoring input Failed to create bus connection: No such file or directory please set right --idtype you input to analysis, The example ID types below are supported:   [1] "ACCNUM"      "ALIA...