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文章 安装picrust2 保存：pkg_resources.DistributionNotFound: The 'cython' distribution was not found and is required by biom-format

...   File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/bin/picrust2_pipeline.py", line 4, in <module>     __import__('pkg_resources').require('PICRUSt2==2.3.0b0')   File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", l...

问题 老师，请问我在做基因组间共线性分析，我的gff 文件，和cds 文件格式如下，如何修改这两个脚本文件get _gene _bed.pl和get _fa _by _bed. pl ，才能提取成功进行下一步分析，谢谢！！

文章 兰花发育中器官发生的时空图谱数据下载示例

....ncbi.nlm.nih.gov/geo/download/?acc=GSE198128&format=file" -O GSE198128_RAW.tar 样本信息 #series_sample_iddevelopmental_stagetissuecodeSRRslideGSM5939574bud1-6FlowerAM559-ST-010001SRR18292617slide1GSM5939575bud7FlowerAM559-ST-010003SRR18292616slide2GSM5939576bud8FlowerAM559-ST-010004SRR182...

文章 从原始测序数据开始分析单细胞数据

#umi_tools whitelist --stdin data/12-22-3_L2_1.fq.gz \ #                    --bc-pattern="(?P<discard_1>CTCTTTCCCT)(?P<cell_1>.{10})(?P<discard_2>ACGACGCTCTTCGAGTGATTGCTTGTGACGCCTT)(?P<cell_2>.{8})(?P<umi_1>.{12})T{4}.*" \ #                    --se...

问题 老师，我在利用perl ../script/get _ene _ exon_ from _ gff.pl in1 时，老是提不出文件，他说十几行不合适但是这个和视频里面的一样，

试了一天了，就是不合适

文章 grep 两个文件内容取交集

...grep 筛选两个文件中共有的信息 N.txt cat N.txtZm00001d003640_P001Zm00001d003640_P002Zm00001d003640_P003Zm00001d010870_P001Zm00001d011034_P001 C.txt cat C.txtZm00001d003640_P001Zm00001d003640_P002Zm00001d003640_P003Zm00001d010870_P001Zm00001d011035_P001 N.txt 和C.txt 的交集grep -F -...

文章 Metadata CRC error detected at xfs _ bmbt _ read _ verify+0x12/0xb0 [xfs], xfs _ bmbt block 0xc9132f8 [ 4.863216] XFS(dm-0): Unmount and run xfs _ repair

Booting` Rocky Linux(5.14.0-427.42.1. e19_4.x86 _64) 9.4(Blue Onyx)' [ 4.862768] XFS (dm-0): Metadata CRC error detected at xfs _ bmbt _ read _ verify+0x12/0xb0 [xfs], xfs _ bmbt block 0xc9132f8 [ 4.863216] XFS(dm-0): Unmount and run xfs _ repair [ 4.863257] XFS(dm-0): First 128 bytes of corr...

问题 pip install RSeQC安装时候报错，是不是底层的库不全？怎么解决呢

...fef3b079fe8c413d05498ca0ae877cffa230564/numpy-1.14.5-cp27-cp27mu-manylinux1_x86_64.whl (12.1MB)     100% |████████████████████████████████| 12.1MB 31.1MB/s  Collecting python-lzo (from bx-python->RseQC)   Downloading http://mi...

问题 我共线比对结果就这么点是为什么

############### Parameters ################ MATCH_SCORE: 50# MATCH_SIZE: 5# GAP_PENALTY: -1# OVERLAP_WINDOW: 5# E_VALUE: 1e-05# MAX GAPS: 25############### Statistics ################ Number of collinear genes: 12, Percentage: 0.05# Number of all genes: 23995#########################################...

文章 单细胞转录组数据挖掘流程记录-LUAD(GSE131907)

...7/suppl/GSE131907%5FLung%5FCancer%5Fraw%5FUMI%5Fmatrix.txt.gz" -O GSE131907_Lung_Cancer_raw_UMI_matrix.txt.gz wget -c "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE131nnn/GSE131907/suppl/GSE131907%5FLung%5FCancer%5Fcell%5Fannotation.txt.gz" -O GSE131907_Lung_Cancer_cell_annotation.txt.gz 样本...