找到约 15 条结果

问题 基因家族分析时用大豆gff文件报错 killed

...ser selected by AGAT: 3 gff3 reader error level1: No ID attribute found @ for the feature: 1    ena_assembly_gap        biological_region      2584646                                                                                    2594645  .  ...

文章 Centos7下Rstudio-service配置教程

...udio/rserver.conf#设置内容如下:# Server Configuration Filersession-which-r=/share/work/biosoft/R/R-v4.0.3/bin/R rsession-ld-library-path=/share/work/biosoft/gcc/gcc-v6.4.0/lib64/:/usr/lib:/usr/lib64:/share/work/biosoft/bamtools/bamtools-v2.4.0/lib/:/share/work/biosoft/gcc/gcc-v6.4.0/lib64/:/...

问题 在01.phylo_tree ,文件格式转换这步,用您的docke或自安装 tassel-5 ,给vcf(23G)文件排序,排成tassel认可的序列,错误如下:Use -Xmx option in start_tassel.pl or start_tassel.batto increase heap size. Included with tassel standalone zip.

问题 Linux中FOR循环做BWA比对报错[main_samview] fail to read the header from "-".

按照重测序课程中的命令 for i in CB2 CB3; do echo  "RUN CMD: bwa mem  Prunus_dulcis.T2T.assembly.fa ${i}_1.fq.gz \ ${i}_2.fq.gz -t 10 -M \ -R '@RG\tID:${i}\tLB:${i}\tPL:ILLUMINA\tSM:${i}' \ |samtools view -bS -h - > ${i}.bam" nohup bwa mem  Prunus_dulcis.T2T.assembly.fa ${i}_...

问题 本地镜像代码运行不了

...gle-cell:v5.0这个镜像,进入后运行代码,会出现bash: cd: $'/work/mypbmc\r': No such file or directory报错,同时运行R,会显示/subset_seurat_obj.r': No such file or directory报错,把RStudio安装到该文件夹下也不行,请问老师该如何解决

文章 WGCNA 中Relationship between module eigengenes图片绘制--plotMEpairs函数

... interpreted as a biologically motivated data reduction scheme that allows for dependency betweenthe resulting components. In contrast, principal component analysis imposes orthogonality between the components.Since modules may represent biological pathways there is no biological reason why modules ...

问题 win10 中docker 下载镜像报错:no matching manifest for windows/amd64 10.0.18362 in the manifest list entries

文章 immune_infiltrates_rnaseq.r RNA-seq 转录组数据免疫侵润分析

...疫侵润分析. In general values should be TPM-normalized ,not log-transformed.optional arguments:  -h, --help            show this help message and exit  -i expset, --expset expset                        input gene expression set matrix from RNA-seq data tsv            ...

问题 老师您好,进行比较基因组分析时候,单拷贝直系同源群为0,后续的分析就出现了报错,这是什么原因,是我选择的基因组不合适还是其他原因。

...homologues 2025-08-08 15:06:41 2025-08-08 15:06:41 : Written final scores for species 0 to graph file 2025-08-08 15:06:44 2025-08-08 15:06:44 : Written final scores for species 3 to graph file 2025-08-08 15:06:46 2025-08-08 15:06:46 : Written final scores for species 1 to graph file 2025-08-08 1...

问题 老师,我正在做泛基因家族分析的结构变异与基因注释部分,出现一些错误

...之后在excel中完成修改,结果如下:之后,#建立注释库 retrieve_seq_from_fasta.pl --format refGene --seqfile ${ref}.fa ${ref}_refGene.txt --out ${ref}_refGene.fa   ,在此过程中出现许多错误并得到最终的${ref}_refGene.fa,请老师帮忙看看是否正常 ...

文章 pennCNV软件linux系统安装

PennCNV安装,官方安装说明:http://penncnv.openbioinformatics.org/en/latest/user-guide/install/ wget https://github.com/WGLab/PennCNV/archive/v1.0.4.tar.gztar xvfz v1.0.4.tar.gzcd kext/make 但是会报错: gcc `perl -MExtUtils::Embed -e ccopts` -fPIC -c -o khmm_wrap.o khmm_wra...

文章 安装picrust2 保存:pkg_resources.DistributionNotFound: The 'cython' distribution was not found and is required by biom-format

...hub.com/picrust/picrust2.git cd Next, install the required dependencies listed here. Install PICRUSt2 into it's own environment using conda: conda env create -f picrust2-env.yaml source activate picrust2 pip install --no-deps --editable . Finally, run the tests to verify the install was succ...

问题 安装docker toolbox 报错:Looks like something went wrong in step ´Looking for vboxmanage.exe´… Press any key to continue…

win10家庭版,CPU虚拟化已经开启。

问题 Error, no sequence for chr1 at xxxxxxxxxxxxxxx/util/gff3_file_to_proteins.pl line 82.

gff3_file_to_proteins.pl用这个工具时报了这个错,是什么原因啊,老师能帮忙解答下吗?

文章 泛基因组可视化

...aths.txt#转换 GFA odgi view -i subregion518.og -g > subregion518.gfa #for  Bandage view#绘图odgi viz -i subregion518.og -o subregion518.png -x 5000 #-z strand Black is forward, red is reverse.#获取FASTA 文件:odgi paths -i subregion518.og -f > subregion518.paths.fasta 结果图如下...