文章 16S多样性分析docker镜像发布及使用

...ggplot2TernaryDESeq2edgeRggtreeveganpheatmap 高级分析相关包 randomForest  机器学习scikit-learn  机器学习circos  圈图绘制Krona  物种丰度圈图LefSe 差异比较分析 注意：以上软件包的列表只是部分举例，实际安装的包还有更多。 扩增子...

问题 向管理员要了metagenomics：v1.0的本地镜像，但是输入 docker load -i metagenomics-v1.0.tar.gz后一直报错 open /var/lib/docker/tmp/docker-import-2588968250/metagenomics/json: no such file or directory

文章 mysql 查询select语句汇总

...','人妖','保密'), cls_id int unsigned default 0, isdelete bit default 0 ); 创建数据： insert into students values (0,'韦少',18,180.00,2,1,0), (0,'小月月',18,180.00,2,2,1), (0,'彭于晏',29,185.00,1,1,0), ...

问题 想要使用plantcare做启动子分析，请问search for care里面输入的序列是什么序列啊？

是CDS还是启动子序列呀？怎杨获得呢？

问题 我使用的服务器，由于文件很多，在使用FOR循环的时候一直出错，老师可以帮忙看一下是哪里有语法错误吗？

/public/singularity exec /public/reseq.sif  for i in FP180000114BR_L01_142 FP180000114BR_L01_143 FP180000114BR_L01_144 FP180000114BR_L01_146 FP180000114BR_L01_147 FP180000114BR_L01_148; do  echo "RUN CMD: fastp --thread 30 --qualified_quality_phred 10 \     --unqualified_percent_limit 50 \ ...

文章 桑基图绘制

...Species)),unique(iris$Class),unique(iris$price))for (i in change){  print(which(change==i))  data[data == i] <-  as.character(which(change==i)-1) }# 绘图所需两个表nodes <-  data.frame("name" = change)links <- apply (data,2,function (x) as.numeric (as.character (x)))links  <-...

问题 UPARSE to pick OTUs in QIIME

...rs filter and chimera detection in "usearch"  and "usearch61"?  And what is more, what is the difference between  "usearch"  and "usearch61", just different version? Is there any methods using the algorithm of UPARSE to pick OTUs in QIIME? Althogh it can do in USEARCH, but I prefer QIIME.    ...

问题 我买了转录组分析的课程，在运行fpkm_and_plot.R脚本时提示Error in library("getopt") : there is no package called ‘getopt’ Execution halted， 我打开R语言安装getopt后仍然显示报错，请教各位大神和组学讲堂的老师

问题 群体遗传学GWAS课程docker很多命令无法运行

...pLDdecay -InVCF  ../00.filter/all.varFilter.vcf.gz -SubPop popid.txt -MaxDist 500 -OutStat ld.stat the Number of subPop samples[found in VCF] is 0 sub Group Population szie is too small, SubGroup sample size: 0 ##begin pair-wise R^2 cal. after filter Remain SNP Number :     0 #% number bin i...

问题 基因家族hmmsearch时提示Failed to open tabular per-dom output file result.txt for writing

基因家族提取用hmmsearch --domtblout result.txt --cut_tc zf-C2H2.hmm  Fvesca_226_v1.1.protein.fa 时提示 Failed to open tabular per-dom output file result.txt for writing 

文章 使用picard对参考序列建索引时报错“java.lang.IllegalArgumentException: File is not a supported reference file type”

...Bit Server VM 19.0.1+10-21; Deflater: Intel; Inflater: Intel; Provider GCS is not available; Picard version: Version:3.0.0 [Fri Oct 13 10:13:20 CST 2023] picard.sam.CreateSequenceDictionary done. Elapsed time: 0.00 minutes. Runtime.totalMemory()=2147483648 To get help, see http://broadinstitute.gith...

问题 我运行遗传群体进化的fst_pi_select_sweep.r这个脚本一直报错Error in dev.off() : cannot shut down device 1 (the null device)，将dev.off()语句改为while (!is.null(dev.list()))  dev.off()依旧报错，怎么解决？

问题 您好，请问一下 Failed to open sequence file GCF-000001735.4-TAIR10.1-protein.faa for reading，hmmsearch出现这种怎么解决呀

您好，请问一下 Failed to open sequence file GCF-000001735.4-TAIR10.1-protein.faa for reading，hmmsearch出现这种怎么解决呀

文章 Dsuite软件安装时编译报错

...alyse(int, int, const std::string&)':Dsuite_common.cpp:124:45: error: 'istream_iterator' is not a member of 'std'  124 |         VCFlineCount = (int)std::count(std::istream_iterator<char>(*vcfFile),std::istream_iterator<char>(),'\n');      |                      ...

问题 输入脚本后报错，python mcscan jcvi 共线性分析报错:ValueError: invalid literal for int() with base 10: ''