找到约 15 条结果

文章 从原始测序数据开始分析单细胞数据

...       --log2stderr > whitelist.txt; # ## Step 3: Extract barcdoes and UMIs and add to read names #umi_tools extract --bc-pattern="(?P<discard_1>CTCTTTCCCT)(?P<cell_1>.{10})(?P<discard_2>ACGACGCTCTTCGAGTGATTGCTTGTGACGCCTT)(?P<cell_2>.{8})(?P<umi_1>.{12})T{4}.*"...

问题 如何修改perl脚本,利用id号抽提gff文件中的位置信息?

...: use Getopt::Long; my %opts; use Data::Dumper; GetOptions (\%opts,"in1=s","in2=s","out=s","h");  if (! defined($opts{in1}) ||! defined($opts{in2})||! defined($opts{out}) || defined($opts{h})){ &USAGE; } open (IN1,"$opts{in1}") || die "open $opts{in1} failed\n"; open (IN2,"$opts{in2}...

文章 串联重复基因 tandem和segmental的区别与联系

...基因家族的时候会有以下疑惑:基因家族串联重复基因tandem和segmental的区别与联系,如何理解基因家族加倍与复制?要回答以上问题,要从基因家族形成方式上去解释: 基因家族的形成方式: 1.染色体組多倍化( Whole genome duplic...

问题 无法链接到docker守护进程

...8:00" level=warning msg="containerd config version `1` has been deprecated and will be removed in containerd v2.0, please switch to version `2`, see https://github.com/containerd/containerd/blob/main/docs/PLUGINS.md#version-header" 然后我按照GitHub的导航操作了还是没解决这个报错...

问题 无法链接到docker守护进程

...8:00" level=warning msg="containerd config version `1` has been deprecated and will be removed in containerd v2.0, please switch to version `2`, see https://github.com/containerd/containerd/blob/main/docs/PLUGINS.md#version-header" 然后我按照GitHub的导航操作了还是没解决这个报错...

文章 从gff文件当中提取对应转录本ID的基因结构信息用于GSDS绘制结构图脚本更新

..._gene_exon_from_gff.pl 用法:perl ../script/get_gene_exon_from_gff.pl -in1 WRKY_domain_new_out_emoved_redundant.txt -in2 Arabidopsis_thaliana.TAIR10.31.gff3 -out gene_exon_info.gff 输出结果(部分): 脚本源代码: use Getopt::Long; my %opts; use Data::Dumper; GetOptions( \%o...

文章 利用qiime2对微生物扩增子测序数据文库根据barcode信息拆分数据

...emux.qza  参考:https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313 课程推荐:微生物扩增子分析课程实操     微生物16S/ITS/18S分析原理及结果解读

文章 熊猫全基因组研究进展

...: [1].   Li R, Fan W, Tian G, Zhu H, He L, Cai J, et al. The sequence and de novo assembly of the giant panda genome[J]. Nature. 2009;463:311. [2].   Hu Y, Wu Q, Ma S, Ma T, Shan L, Wang X, et al. Comparative genomics reveals convergent evolution between the bamboo-eating giant and red panda...

问题 meme分析后显示error at: maxw

...name of background Markov model file[-revcomp]allow sites on + or - DNA strands[-pal]force palindromes (requires -dna)[-maxiter <maxiter>]maximum EM iterations to run[-distance <distance>]EM convergence criterion[-psp <pspfile>]name of positional priors file[-prior dirichlet|dmix|m...

问题 同学,你这个问题解决了吗

...soft/MCScanX/MCScanX/MCScanX ST_AT 得到以下结果 Reading BLAST file and pre-processingGenerating BLAST list170 matches imported (1 discarded)44 pairwise comparisons0 alignments generatedPairwise collinear blocks written to ST_AT.collinearity [0.013 seconds elapsed]Writing multiple syntenic bl...

文章 小麦TCP基因家族分析文献解读及原文献下载

...家族分析原文献下载(点此链接):Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.).pdf 小麦TCP基因家族分析文献解读 日常科研中你我经会常遇到看不懂的图表,不会挖...

问题 conet构建网络图参数设置

...法部分有提到“Pairwise correlations were determined using Pearson and Spearman correlations as described previously. Only relatively large correlation coefficients (ρ > 0.8 and p<0.01) detected with both methods (Pearson and Spearman) were included into network analyses to minimize fal...

文章 perl中读取和写出Excel的包,xls,和xlsx

...); # Add a worksheet $worksheet = $workbook->add_worksheet(); #  Add and define a format $format = $workbook->add_format(); # Add a format$format->set_bold();$format->set_color('red');$format->set_align('center'); # Write a formatted and unformatted string, row and column notation...

问题 Agilent单色背景校正,Error in if (alpha

...ted Array 2 corrected Array 3 corrected Array 4 corrected Array 5Error in if (alpha <= 0) stop("alpha must be positive") :    missing value where TRUE/FALSE needed In addition: Warning message: In log(out$alpha) : NaNs produced

问题 GWAS分析中提示Error in hclust(d, method = method) : NA/NaN/Inf in foreign function call (arg 11) Calls: pheatmap -> cluster_mat -> hclust Execution halted

Error in hclust(d, method = method) :   NA/NaN/Inf in foreign function call (arg 11) Calls: pheatmap -> cluster_mat -> hclust Execution halted