问题 扩增子：真菌ITS分析unite不匹配

...目前unite没有下载到最新的参考序列  qiime deblur denoise-other \    --i-demultiplexed-seqs demux-joined-filtered.qza \    --i-reference-seqs $dbdir/qiime2/unite_ver9_99_25.07.2023-Q2-2023.7.qza \    --p-trim-length -1 \    --p-sample-stats \    --p-jobs-to-start 10 \ ...

问题 Use of uninitialized value $id in lc at /share/work/biosoft/perl/perl-5.22.1/lib/site_perl/5.22.1/AGAT/OmniscientI.pm会影响结果吗

1 warning messages: Use of uninitialized value $id in lc at /share/work/biosoft/perl/perl-5.22.1/lib/site_perl/5.22.1/AGAT/OmniscientI.pm line 1198, <$fh> line 3385475. 1 warning messages: Use of uninitialized value $id in lc at /share/work/biosoft/perl/perl-5.22.1/lib/site_perl/5.22.1/AGAT...

问题 老师，我在windows10系统下安装了docker desktop，但是一直提示out of memory，怎么办？

问题 老师，您好！key driver analysis的参考文献如下。请您帮我查查该分析方法的R语言包在哪里下载？

Identification of Key Causal Regulators in Gene Networks . Bin Zhang and Jun Zhu .Proceedings of the World Congress on Engineering 2013 Vol II, .

文章 压缩或解压软链接文件，gzip或gunzip报错Too many levels of symbolic links

...的文件，想对它进行压缩，但是却报错 gzip: Too many levels of symbolic links 通过看gzip说明文档 可以使用 -c 标准输出到别的文件，报错就解决了 gzip -c raw.filtered.vcf > raw.filtered.vcf.gz 二、gunzip报错 同样的情况，如果对软...

文章 基于R实现统计中的检验方法---T检验

...  sites  t = -0.93574, df = 9, p-value = 0.3738alternative hypothesis: true mean is not equal to 4.595 percent confidence interval: 4.230016 4.611984sample estimates:mean of x     4.421 p=0.3738>0.05,认为所抽样水体的含氧量与多年平均值无显著差...

文章 Dsuite软件安装时编译报错

...amp;)':Dsuite_common.cpp:124:45: error: 'istream_iterator' is not a member of 'std'  124 |         VCFlineCount = (int)std::count(std::istream_iterator<char>(*vcfFile),std::istream_iterator<char>(),'\n');      |                                             ...

问题 juicer 报错： Failure during chimera handling of

...2.fastq.gz.sam source line number 298***! Failure during chimera handling of /home/data/t090519/JF/20.Jui/juicer/work/splits/JF2.fastq.gz附件为nohup.out信息，报错在最后几行nohup.out

问题 关于单倍型分析的问题

老师您好！ 不太明白这句话：A haplotype network of SD6. The haplotype network was constructed based on SNPs at the SD6 gene region using 3,000 rice genome data。这个snp是指vcf文件中0|1,0|0,这种数据吗？还是来自一条染色体某区段的snp位点的组合？ 我在ht...

文章 保姆式教程！手把手教你使用Simplot，绘制高大上的序列相似性图片

...: Proposal for Posaliviridae as a new family in Picornavirales. Virus Res. 2021 Oct 2;303:198501. doi: 10.1016/j.virusres.2021.198501. Epub 2021 Jul 9. PMID: 34252491. 版本：SimPlot 3.5.1

文章 igraph 包安装错误

... * removing ‘/share/work/biosoft/R/R-v3.4.0/lib64/R/library/igraph’ The downloaded source packages are in         ‘/tmp/Rtmply12uv/downloaded_packages’ Updating HTML index of packages in '.Library' Making 'packages.html' ... done 错误解决,临时不让他加载这些模块...

问题 WGCNA中绘制性状与模块相关性图出现colorMatrix[, c] : incorrect number of dimensions

之前完全没问题，绘图时出现错误 数据内容如下： 性状数据内容： textMatrix矩阵内容： 实在是不知道到底哪个矩阵的维度不对了...

问题 如何用荧光定量数据绘制热图？

...n profiles of PtSAUR genes in different tissues as determined by qRT-PCR. There were three biological and three technicalreplicates performed for each sample. The relative expression levelswere evaluated by 2-ΔΔCT. GraphPad software was used for the statistical analyses. 作者是用的2-ΔΔCT...

文章 Nature Medicine同期8篇论文，聚焦人工智能在医学领域的应用

...BioWorld列举并介绍了这8篇论文： 1 High-performance medicine: the convergence of human and artificial intelligence 高效医学：人类与智能的融合 作者：Eric J. Topol；单位：Scripps研究所 通过在所有部门中使用标记的大数据以及显著增强的计算...

文章 GATK报错| this implies a problem with the file locking support in your operating system

... was reached while trying to acquire a lock on file raw.vcf.tmp.idx. Since the GATK uses non-blocking lock acquisition calls that are not supposed to wait, this implies a problem with the file locking support in your operating system. ##### ERROR -----------------------------------------------------...