找到约 15 条结果

问题 基因组注释Braker里面的报错

...f3 --skip_fixing_broken_genes” 报错“# Mon Aug 12 20:10:06 2024: Log information is stored in file /work/work/xsyj/genome_annotation/03.Braker/braker/braker.log ERROR in file /share/work/biosoft/BRAKER/latest/scripts//braker.pl at line 6666 Failed to execute: perl /share/work/biosoft/GeneMar...

文章 计算肿瘤纯度和免疫评分 R包总结

...ypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR, and NF1."Cancer Cell 2010, 17:98-110. [1] https://www.genepattern.org/modules/docs/ABSOLUTE/2 [2] http://software.broadinstitute.org/cancer/cga/absolute_run [3] http://software.broadinstitute.org/cancer/software/genep...

文章 利用NCBI当中的CD-search工具预测基因的保守结构域

...at represent various confidence levels (specific hits, non-specific hits) and domain model scope (superfamilies, multi-domains). They can be seen in both the Concise display and Full display, except for non-specific hits, which are shown only in the Full Display.PSSM-IDA PSSM ID is the unique...

文章 植物单细胞转录组文章

...own in gel conditions, 2 wilde type grown in non-compacted soil conditions and 2 wild type grown in compacted soil conditions. scRNA-seq of 2 kitaake grown in gel conditions, 2 Kitaake grown in non-compacted soil conditions. 单细胞转录组揭示玉米根的热胁迫反应机制 Wang, ...

文章 RIP实验技术

...tochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22(2):163-174(2016).         3.Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes. Nucleic Acids Res(2017).

问题 RNAseq有参转录组数据自主分析课程 ,运行建立基因组索引命令时,报错,无法获取gene_length.txt文件,请问是什么原因?如何处理呢?

...genome.fasta Toona_sinensis.gene.gff 报错信息如下:get gene length and gene.bed from gtf: RUN CMD: python /work/TS/scripts/get_gene_length_from_gtf.py -g Toona_sinensis.gene.gtf -p gene_length Traceback (most recent call last):   File "/work/TS/scripts/get_gene_length_from_gtf.py", line ...

文章 lefse 运行报错python3中

...s no attribute 'rownames' 解决办法,降低版本: rpy2 (3.5.10) and numpy (1.24.3)

文章 重测序群体遗传进化分析之—进化树构建

...多不同的模型计算初始简约树的逻辑概率,并产生Akaike information criterion (AIC),* corrected Akaike information criterion* (AICc), and* the Bayesian information criterion* (BIC)三个结果标准值,通常ModelFinder选择BIC分数最低的模型(当然也可以指定AIC和AI...

问题 请教老师,Gemma分析时的报错问题?

[root@iZ2e]# ./gemma-0.98.5-linux-static-AMD64 -bfile c -k output/result1.sXX.txt -lmm 1 -p p.txt -c c.txt GEMMA 0.98.5 (2021-08-25) by Xiang Zhou, Pjotr Prins and team (C) 2012-2021 ERROR: Enforce failed for file_kin output/result1.sXX.txt: open file in src/param.cpp at line 934 in CheckParam

问题 WGCNA-MAD

...at is in the data set dim(datExpr) ##################check missing value and filter ################### datExpr = t(datExpr[order(apply(datExpr,1,mad), decreasing = T)[1:5000],]) dim(datExpr) 根据上面的代码,我想把1.8w个基因缩减下,结果在进行MAD筛选前5k个基因的...

文章 4个样品重测序发PlosOne

...高深度重测序,四个品种中三个高抗白粉病( Edisto, MR-1, and PMR5: resistant),一个易感白粉病(SCNU1154: susceptible;),得到测序reads与参考基因组比对,查找SNP,Indel,SV等变异信息,并对变异信息进行质控过滤,变异信息在染色体...

问题 我的Conda 根本安装不成功软件,比如UMI tools ,怎么办

...es.DistributionNotFound: The 'umi-tools==0.2.3' distribution was not found and is required by the application

问题 ANNOVAR变异注释报错:Erro: invalid record found in exonic_variant_function file (exonic format error)

...use --nopolish to change this behavior) NOTICE: Running with system command <convert2annovar.pl  -includeinfo -allsample -withfreq -format vcf4 /work/bdorsalis/4.snp_indel/var_qc/all.clean.snp.vcf.gz > /work/bdorsalis/5.var_ann/test/snp.avinput> NOTICE: Finished reading 840 lines from ...

问题 请问一下做WGCNA时,出现这种问题如何解决呢?用的是课程的数据

traitColors = numbers2colors(datTraits, signed = FALSE) Warning message: In numbers2colors(datTraits, signed = FALSE) : (some columns in) 'x' are constant. Their color will be the color of NA. > # Plot the sample dendrogram and the colors underneath.

文章 VG 泛基因组call 变异

#this script report the commands used to call variants from the 16 Illumina WGS barn swallow individuals.#for steps 1-3, this github solution was followed: https://github.com/vgteam/vg/issues/3411#for steps 4-9, the section "Calling the graph by first splitting into components" of this tutorial was ...