...了冷胁迫对照处理3天后093CK、093T、120CK和120T四组植株的净光合速率 (Pn)、蒸腾速率 (E) 、气孔导度 (C)和MDA浓度。 b. 得出结论:在冷胁迫处理下,两品种植株的净光合速率、蒸腾速率、气孔导度都有明显降低...
...fasta.clean -u all_transcripts.fasta --CPU $threads --ALIGNERS blat,minimap2 --TRANSDECODER --TDN tdn.accs --MAX_INTRON_LENGTH 1000000 这步时报错, minimap2 TRINITY_GG_8151_c3_g1_i1 122192 1151017 chr9_RagTag 1 + ? OK 30632781-30633198 99.8 100 41716.4 orient(a+/s?) align: 30632781(1)-306...
老师给的命令 hisat2_extract_splice_sites.py chr22_with_ERCC92.gtf > splicesites.tsvhisat2_extract_exons.py chr22_with_ERCC92.gtf > exons.tsvhisat2-build -p 8 --ss splicesites.tsv --exon exons.tsv chr22_with_ERCC92.fa chr22_with_ERCC92 请问这样比直接 hisat2-build好在什么...
...想请教您个问题,我有两年数据,两年合在一起的数据和20220年数据分析正常,但是2025年的数据分析异常,用tassel分析了一般线性模型和混合线性模型,用GEMMA做了一般线性模型,结果都如图所示,运算脚本 tassel GLM模型:ohup ru...
...--vcf sample.vcf --weir-fst-pop population_1.txt --weir-fst-pop population_2.txt --out P_1_2 --fst-window-size 10000 --fst-window-step 1000 然后选取结果中top1%的区段和我基因区段做overlap,然后去看是否受到选择是对的么? 另外,如果用π和tajimaD都是需要分...
...usr/local/HDF_Group/HDF5/1.8.18/examples": No such file or directory make[2]: *** [install] Error 1 make[2]: Leaving directory `/home/LTT/install/lordec-src_0.9/gatb-core-1.4.1/gatb-core/build' make[1]: *** [gatb_v1.4.1/include/gatb/gatb_core.hpp] Error 2 make[1]: Leaving directory `/home/LTT/in...
...,其具体用法如下: Tool: bedtools nuc (aka nucBed)Version: v2.25.0Summary: Profiles the nucleotide content of intervals in a fasta file.Usage: bedtools nuc [OPTIONS] -fi <fasta> -bed <bed/gff/vcf>Options: -fi Input FASTA file -bed BED/GF...
...fml, exprSet) cox_sum <- summary(gcox) HR <- round(cox_sum$coefficients[,2],2) PValue <- round(cox_sum$coefficients[,5],4) CI <- paste0(round(cox_sum$conf.int[,3:4],2),collapse='-') Uni_cox <- data.frame('Characteristics' = x, 'Hazard Ratio' = HR, ...
...TCH_SIZE: 5# GAP_PENALTY: -1# OVERLAP_WINDOW: 5# E_VALUE: 1e-05# MAX GAPS: 25############### Statistics ################ Number of collinear genes: 12, Percentage: 0.05# Number of all genes: 23995############################################ Alignment 0: score=277.0 e_value=0 N=6 NW_014685782.1&N...
...用准备文件: 1. genome.softmasked.fa # 软掩码之后的基因组2. all_transcripts.fasta # 有参、无参的转录组预测转录本;以及est.fa序列3. all_transcripts.fasta.clean # 2的序列进行seqclean过滤之后的结果4. FL_accs.txt # 全长转录本序列的ID名称5. t...
...言叫做:Shell 脚本,这个脚本语言可以帮助我们简化很多工作,例如批量运行一些命令等,所以还是很有必要学习它的基本用法的,一个简单的 hello.sh 脚本像下面这样。 #!/bin/bash echo "Hello World" 第一行 #!/bin/bash 标识该 Shell...
...ta所以跳过质控直接做map,修改了代码如下 for i in SRR9113321 SRR9113322 SRR9113323 SRR9113324 SRR9113325 SRR9113326 SRR9113327 SRR9113328 SRR9113329 SRR9113330 SRR9113331 SRR9113332 SRR9113333; do echo "RUN CMD: hisat2 -p 1 --rg-id=${i} --rg SM:${i} --rg LB:${i} --rg PL:ILLUMINA \ ...
...sit.co/download/rstudio-server/ root账号安装: wget https://download2.rstudio.org/server/centos7/x86_64/rstudio-server-rhel-2022.07.2-576-x86_64.rpm sudo yum install rstudio-server-rhel-2022.07.2-576-x86_64.rpm 启动R 服务: rstudio-server start #启动RStudiorstudio-server status #...
tail -n+2 $datafile/metadata_WBBW_all.txt | cut -f1 | rush -j 4 'java -jar /share/work/biosoft/Trimmomatic/Trimmomatic-0.39/trimmomatic-0.39.jar PE \ -threads 6 \ "$datafile"/{}_1.fq.gz "$datafile"/{}_2.fq.gz \ "$workdir"/1.host_del_and_qc/kneaddata/{}_forward_paired.fq.gz "$workdir"/1.h...
...NA成熟序列;Rhi_hairpin.fa 是物种miRNA前体序列; perl miRDeep2.pl Total_reads_collapsed.fa GCA_000334115.1_Rhisol_AG1IA_1.0_genomic.fa Total_reads_collapsed_vs_genome.arf none -d -g 100000 -l 500 -m 11 -v -P -n d 报错: The debug file is called error.output.mrd_04_09_2023_t_1...