基于HIC和hifi数据，hifiasm 组装了基因组，然后bwa sampe xblygenome.final.fa sample_R1.sai sample_R2.sai XBLYyehic_R1.clean.fastq.gz XBLYyehic_R2.clean.fastq.gz > sample.bwa_aln.sam正常，得到78G文件，export PATH=$PATH:/share/biosoft/bedtools/ALLHIC/scripts/， PreprocessSAMs.pl   hicxbly_aligned.bam xblygenome.final.fa MBOI正常， 运行 filterBAM_forHiC.pl hicxbly_aligned.REduced.paired_only.bam xblysample.clean.sam，生成的文件为零，是什么原因 用$samtools view -c hicxbly_aligned.REduced.paired_only.bam 查看，有1465429722reaads