16S课程 02.feature_table这一节课中 dada2方法无法生成数据

attachments-2026-04-vNpUO6zJ69d1a9003dff9.png我输入的是这个指令:

qiime dada2 denoise-paired \

    --i-demultiplexed-seqs ../01.import_data/demux.qza \

    --p-n-threads 2 \

    --p-trim-left-f 0 --p-trim-left-r 0 \

    --p-trunc-len-f 300 --p-trunc-len-r 260 \

    --o-table dada2-table.qza \

    --o-representative-sequences dada2-repset-seqs.qza \

    --o-denoising-stats dada2-stats.qza

报错:

Plugin error from dada2:

  An error was encountered while running DADA2 in R (return code 1), please inspect stdout an                            d stderr to learn more.

Debug info has been saved to /work/my_ampliseq/tmp/qiime2-q2cli-err-5s4z89nu.log


这个参数设置问题,课程中讲述不明确,并且课程中让我们这么设置--p-trunc-len-f 150 --p-trunc-len-r 140 导出的table后续分析用不了

--p-trim-left-f 0 --p-trim-left-r 0 \

    --p-trunc-len-f 300 --p-trunc-len-r 260 \


想请老师帮忙解答一下!

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