植物线粒体组装unicycler软件里的spades报错

安装教程里的代码运行,使用unicycler软件时报错,报错信息如下

== Error ==  system call for: "['/share/work/biosoft/SPAdes/SPAdes-3.15.4-Linux/bin/spades-core', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/config.info', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/isolate_mode.info']" finished abnormally, OS return value: -9

None


======= SPAdes pipeline finished abnormally and WITH WARNINGS!


=== Error correction and assembling warnings:

 * 0:06:44.998  1783M / 18G   WARN    General                 (kmer_coverage_model.cpp   : 218)   Too many erroneous kmers, the estimates might be unreliable

 * 0:07:10.518  1785M / 18G   WARN    General                 (kmer_coverage_model.cpp   : 327)   Valley value was estimated improperly, reset to 3

 * 0:07:10.523  1785M / 18G   WARN    General                 (kmer_coverage_model.cpp   : 366)   Failed to determine erroneous kmer threshold. Threshold set to: 3

======= Warnings saved to /work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/warnings.log


=== ERRORs:

 * system call for: "['/share/work/biosoft/SPAdes/SPAdes-3.15.4-Linux/bin/spades-core', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/config.info', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/isolate_mode.info']" finished abnormally, OS return value: -9


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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

输入的数据可能有问题,纠错步骤抱错了。

再看看计算资源是否够,内存是不是够;

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