老师,关于PASApipeline配置的问题

运行/ldata/xiongmei/ceshi/PASApipeline.v2.4.1/Launch_PASA_pipeline.pl -c pasa.annotationCompare.Template.txt -g wjsy_rm_adj.fa -t transcripts.fa -A -L --annots evm.out.gff3,总是报错DBI connect('database=wjsy11;host=localhost','shehb',...) failed: Access denied for user 'shehb'@'localhost' (using password: YES) at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/PerlLib/DB_connect.pm line 72.

Cannot connect to wjsy11: Access denied for user 'shehb'@'localhost' (using password: YES) at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/scripts/Load_Current_Gene_Annotations.dbi line 71.

Error, cmd: /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/scripts/Load_Current_Gene_Annotations.dbi -c pasa.annotationCompare.Template.txt -g wjsy_rm_adj.fa -P evm.out.gff3  > pasa_run.log.dir/output.annot_loading.69620.out died with ret 65280 No such file or directory at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/PerlLib/Pipeliner.pm line 187.

Pipeliner::run(Pipeliner=HASH(0x1a2a6b0)) called at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/Launch_PASA_pipeline.pl line 1044attachments-2023-04-OqQUXQPl644600db3d306.png

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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

建议用我们的docker镜像,里面都设置好了。自己配置PASA可以参考PASA官方网站;

不同的服务器mysql安装环境不一样,我这边不好帮你查原因

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  • 熊梅 提出于 2023-04-24 12:09

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