在用ALLHIC软件挂载的时候，用如下命令：PreprocessSAMs.pl   sample.bwa_aln.sam draft.asm.fasta DPNII。但是运行的时候，出现报错。报错如下：

 Wed Jul 23 15:32:05 2025: PreprocessSAMs.pl: samtools view -bS sample.bwa_aln.sam -o sample.bwa_aln.bam

      2 /share/work/biosoft/ALLHiC/ALLHiC/scripts/PreprocessSAMs.pl sample.bwa_aln.sam draft.asm.fasta DPNII

      3

      4 Wed Jul 23 16:18:33 2025: PreprocessSAMs.pl: /share/work/biosoft/ALLHiC/ALLHiC/scripts/make_bed_around_RE_site.pl

      5

      6 make_bed_around_RE_site.pl

      7

      8 Find all occurrences of a motif in a genome.  Make a 'POS' file listing these occurrences, and also a BED file re

      9

     10 SYNTAX: make_bed_around_RE_site.pl <fasta> <motif> <range>

     11 fasta:  A fasta file representing a genome (reference or draft assembly.)

     12 motif:  A motif, typically a restriction site sequence (e.g., HindIII = AAGCTT, NcoI = CCATGG, Dpn1 = GATC).

     13 range:  A number representing how many bp around the sequence to include.  Recommend 500 based on Yaffe & Tanay,

     14

     15 OUTPUT FILES:

     16 <fasta>.near_<motif>.<range>.bed

     17 <fasta>.near_pos_of_<motif>.txt

     18

     19 Wed Jul 23 16:18:33 2025: PreprocessSAMs.pl: bedtools intersect -abam sample.bwa_aln.bam -b draft.asm.fasta.near_

     20 Error: Unable to open file draft.asm.fasta.near_.500.bed. Exiting.

     21 Wed Jul 23 16:18:33 2025: PreprocessSAMs.pl: samtools view -F12 sample.bwa_aln.REduced.bam -b -o sample.bwa_aln.R

     22 [main_samview] fail to read the header from "sample.bwa_aln.REduced.bam".

     23 Wed Jul 23 16:18:33 2025: PreprocessSAMs.pl: samtools flagstat sample.bwa_aln.REduced.paired_only.bam > sample.bw

     24 [E::hts_open_format] Failed to open file "sample.bwa_aln.REduced.paired_only.bam" : No such file or directory

     25 samtools flagstat: Cannot open input file "sample.bwa_aln.REduced.paired_only.bam": No such file or directory