找到约 15 条结果

问题 成功安装WGCNA包后,调用时报错

...urce* package 'GO.db' ... ** R ** inst ** byte-compile and prepare package for lazy loading ** help *** installing help indices converting help for package 'GO.db' finding HTML links ... ºÃÁË GOBASE html GOBPANCESTOR htm...

文章 单细胞转录组-inferCNV拷贝数变异分析介绍

...he root of the dendrogram is linked to the root of each type's dendrogram, which leads to having all of them on the same level). The first color column is of a single color as k_obs_groups is not used when clustering by annotation. The second color column indicates the annotation matching to each ce...

问题 在RStudio中安装WGCNA包后调用WGCNA包提示错误

...ckage:stats’:     hclust Error: package or namespace load failed for ‘WGCNA’ in rbind(info, getNamespaceInfo(env, "S3methods")):  number of columns of matrices must match (see arg 2)

文章 SCTransform ,Error: useNames = NA is defunct

...r: countsvst.flavor='v2' set. Using model with fixed slope and excluding poisson genes.Variance stabilizing transformation of count matrix of size 20445 by 2713Model formula is y ~ log_umiGet Negative Binomial regression parameters per geneUsing 2000 genes, 2713 cellsError: useNames = NA is defunct....

问题 omicsclass/gene_family同时有latest和V1.0.1两个版本,挂载时出错

Error response from daemon: pull access denied for it, repository does not exist or may require 'docker login': denied: requested access to the resource is denied. 麻烦老师解答一下,之前听docker blast那里挺顺利的,在基因家族分析课这里卡住了

文章 igraph 报错: libglpk.so.36: cannot open shared object file: No such file or directory

** demo ** inst ** byte-compile and prepare package for lazy loading ^A** help *** installing help indices *** copying figures ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location Error: package or namespace load failed fo...

问题 运行代码的时候出错,我确认文件都存在,报错代码如下所示

...ht, SofaAnstieg, Hauotdiagnos... lgl  (1): Groupf ℹ Use `spec()` to retrieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in `select()`: ! Can't subset columns with `metaData[, 1, drop = T]`. ✖ Can...

问题 分歧时间算不出来结果

...    RootAge = <0.9  * safe constraint on root age, used if no fossil for root.          model = 4    * 0:JC69, 1:K80, 2:F81, 3:F84, 4:HKY85          alpha = 0.5    * alpha for gamma rates at sites          ncatG = 5    * No. categories in discrete gamma      cle...

问题 reseqv2.0镜像annovar报错

...system command <convert2annovar.pl  -includeinfo -allsample -withfreq -format vcf4 /work/my_reseq/4.snp_indel/var_qc/pyb4.clean.vcf.gz > /work/my_reseq/5.var_ann/snp.avinput> NOTICE: Finished reading 656047 lines from VCF file NOTICE: A total of 656008 locus in VCF file passed QC thresho...

问题 执行ALLHiC分组不成功

...ct sample.clean.bam draft.asm.fasta --RE GATC ## 排序和方向优化 for K in `seq  1 $k`; do     allhic optimize sample.clean.counts_GATC.${k}g${K}.txt sample.clean.clm done 报错## 排序和方向优化 [root@90227036c754  20:53:04 /work/my_LQ28473/30.ALLHiC1]# for K in `seq  1 $k...

问题 RNA 速率分析Pseudotime vs latent time

I am doing scRNA-Seq analysis, and I am doing RNA velocity and pseudotime analyses as part of my analysis pipeline. Now, RNA velocity is carried out using scvelo, and this package generates a metric known as latent time. I am having a very had time seeing the difference between latent time and stand...

文章 筛选编码蛋白的CDS序列

...out_dir)count=0total=0output_handle = open(dout+'/'+args.prefix+'.fa', "w")for rec in SeqIO.parse(args.fasta, "fasta"):    seq=str(rec.seq)    #seq=seq.upper()    total+=1    if(len(seq)>=args.len and re.search("^ATG",seq,re.I) and len(seq)%3==0):        if re.search('TAG$',seq,re.I) ...

问题 python rpy2 报错

...38:39)[GCC 6.4.0] on linuxType "help", "copyright", "credits" or "license" for more information.>>> import rpy2.robjects as robjError: package or namespace load failed for ‘methods’ in dyn.load(file, DLLpath = DLLpath, ...): unable to load shared object '/share/work/biosoft/R/R-v4.0.3/...

文章 NCBI 基因组文件下载——datasets

...3 --filename GCA_027406505.1.zip 3. 帮助信息 ./datasets --helpData Retrieval Commands  summary     Print a data report containing gene, genome or virus metadata  download    Download a gene, genome or virus dataset as a zip file 下载的结果是:ncbi_dataset.zip  rehydrate   Re...

文章 perl自动获取网页上的信息

... to that subroutine.     # Inside a subroutine, @_ is the default array for the array operators push, pop, shift, and unshift.     my $url = 'http://www.taobao.com';    die "no url param!\n" unless $url;     # create LWP::UserAgent object     my $ua = LWP::UserAgent->new;     # set ...