找到约 15 条结果

问题 用GATK call SNP时报错

...raversal by intervals was requested but some input files are not indexed. Please index all input files: samtools index SRR3274663.new.sorted.bam Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace. 这是...

问题 用R包TCGAbiolinks下载TCGA的miRNA数据报错

...the download process (we might had a connection problem with GDC server). Please run this function it again. Try using method = `client` or setting files.per.chunk to a small number. 把files.per.chunk设置成很小,依然这样报错。 请问该怎么解决呢?

问题 TCGAbiolinks GDCprepare使用问题 提示找不到文件?

...or in GDCprepare(query) : I couldn't find all the files from the query. Please check if the directory parameter is right or ‘GDCdownload’downloaded the samples. 我尝试了改路径,下载到我指定的文件夹(因为GDCprepare是默认的GDCdata文件夹)但每次都下不到里面...

文章 R was not built as a library python 中 rpy2 报错

...新下载最新版本的R 3.4.2并编译安装:./configure --prefix=<YOUR_R_HOME> --enable-BLAS-shlib --with-blas --with-lapackmake && make install 最重要的是如果R安装到个人路径下,记得添加环境变量: export R_HOME=path-to-R export R_LIBS=$R_HOME/lib64/R/libr...

问题 转录组KEGG富集分析出现问题。

...hsa --idtype ENSEMBL --totype ENTREZID --> Q&A for bioinformatics, please visit the website: www.omicsclass.com --> Recommended to learn R language: --> https://study.163.com/course/introduction/1209073807.htm?share=1&shareId=1030291076   Registered S3 method overwritten by 'e...

文章 NCBI下载的10X单细胞数据只有read1和read2如何读入cellranger

....join(args.output_dir, new_filename)                        # Check if target exists            if os.path.exists(new_file):                if args.force:                    os.remove(new_file)                else:                    print(f"War...

话题 gff3_file_to_proteins.pl

问题 单细胞测序数据差异基因KEGG富集分析

...gnoring input Failed to create bus connection: No such file or directory please set right --idtype you input to analysis, The example ID types below are supported:   [1] "ACCNUM"      "ALIAS"       "ENTREZID"    "ENZYME"      "EVIDENCE"     [6] "EVIDENCEALL" "GENENAME"    "GO"...

问题 R包安装错误 cp: cannot stat ‘hdf5/src/.libs/libhdf5.a’: No such file or directory make: *** [copying] Error 1 ERROR: compilation failed for package ‘Rhdf5lib’

...ew/Rhdf5lib/lib/" cp: cannot stat ‘hdf5/src/.libs/libhdf5.a’: No such file or directory make: *** [copying] Error 1 ERROR: compilation failed for package ‘Rhdf5lib’ * removing ‘/share/work/biosoft/R/R-v4.0.3/lib64/R/library/Rhdf5lib’ * installing *source* package ‘BiocStyle’ ......

问题 gff3_file_to_proteins.pl 报错

求助这是哪一步出问题了呢

问题 gff3_file_to_proteins.pl 报错

老师,统一了格式还是会出现,这种情况会是哪里有问题呢

问题 GWAS分析绘制进化树遇到下面问题是什么意思,请问怎么解决?

...: ignoring environment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Registered S3 methods overwritten by 'treeio':   method          ...

文章 Pacbio三代全长转录组(Iso-Seq ) 分析软件Cogent 的安装

...naconda.org/timcera pulp 3. 安装Cogent, 目前的版本是v3.5, cd <your_dir> git clone https://github.com/Magdoll/Cogent.git cd Cogent # 目前最新版本是v3.5 git checkout tags/v3.5 git submodule update --init --recursive cd Complete-Striped-Smith-Waterman-Library/src make export LD...

文章 textshaping L :1:19: fatal error: hb-ft.h: No such file or directory

... harfbuzz fribidi (OSX)If harfbuzz freetype2 fribidi is already installed, check that 'pkg-config' is in yourPATH and PKG_CONFIG_PATH contains a harfbuzz freetype2 fribidi.pc file. If pkg-configis unavailable you can set INCLUDE_DIR and LIB_DIR manually via:R CMD INSTALL --configure-vars='INCLUDE_DI...

文章 提取 VCF 文件中的基因型信息

...命令可以提取所有样本的基因型: bcftools query -f '%GT\n' your_file.vcf 还可以根据需要自定义输出格式。 2.VCFtools VCFtools 是一个流行的 VCF 文件处理工具集,它提供了许多功能,包括提取基因型信息。你可以使用 --extract-FORMAT-info ...