找到约 15 条结果

问题 bioconductor常用包 安装

我装R是3.6.1,安装bioconductor常用包一直出问题。 > install.packages("BiocManager")WARNING: Rtools is required to build R packages but is not currently installed. Please download and install the appropriate version of Rtools before proceeding:https://cran.rstudio.com/bin/windows...

问题 ggplot,超过12种颜色,scale_color_brewer(type="seq",palette="Paired", direction=-1)+ 颜色不够用

...19个样本,但调用scale_color_brewer时只有12种颜色,导致我点线图里有七个没有颜色,这种情况应该怎么办? p_line_point = ggplot(dat, aes(x=dat[,2],y=dat[,3],shape=dat[,1],color=dat[,1]))+  geom_line(size=1)+  scale_color_brewer(type="seq",palette="Paired",...

问题 Get "https://theme-kit-access.shopifyapps.com/cli/meta.json": dial tcp 185.146.173.20:443: connectex: A connection attempt...

关于shopify与本地服务器之间连接报错,求各位大佬指教指教!!

问题 Error, no sequence for chr1 at /wcr/TransDecoder/util/gff3_file_to_proteins.pl line 82.

你好,你运行脚本时候遇到问题了吗?Error, no sequence for chr1 at xxxxxxxxxxxxxxxx/util/gff3_file_to_proteins.pl line 82.

文章 R包hdf5r安装失败 SeuratDisk 安装失败

...iled for package ‘hdf5r’ 解决办法: 1. 下载hdf5-1.8.13源码 wget https://support.hdfgroup.org/ftp/HDF5/releases/hdf5-1.8/hdf5-1.8.13/src/hdf5-1.8.13.tar.bz2 2. 编译安装 tar xjf hdf5-1.8.13.tar.bz2 cd hdf5-1.8.13 ./configure --prefix=$HOME/.local/bin/hdf5-1.8.13 make ...

问题 tophat2建立索引报错:Error: gtf_to_fasta returned an error.

tophat建立索引时候出现报错; "/share/work/biosoft/tophat/tophat-2.1.1/bin/tophat2" -G Sspon.v20180123.gtf --transcriptome-index=transcriptome_data/known Sspon.HiC_chr_asm [2019-04-28 05:49:42] Building transcriptome files with TopHat v2.1.1--------------------------------------------...

文章 GATK|Timeout of 30000 milliseconds was reached while trying to acquire a lock on file raw.vcf.tmp.idx.

...法:https://www.omicsclass.com/article/2194 这里还有一个需要做步骤,就是先raw.vcf.tmp.idx文件找到并删除,一般情况下这个文件是空。 然后再在命令行中添加-disable_auto_index_creation_and_locking_when_reading_rods 这个参数。并运行命令...

问题 Error in UseMethod("groupOTU") : no applicable method for 'groupOTU' applied to an object of class "NULL" Calls: groupOTU Execution halted

各位老师,我在绘制进化树过程中,使用命令ggtree.r -t fasttree.nwk -f $GROUP -g Group -n fasttree,运行后报错,出现下图报错,请问老师,我应该怎么处理呢?

问题 使用tassel软件对vcf文件排序时出现ERROR net.maizegenetics.plugindef.AbstractPlugin - java.lang.IllegalArgumentException: AlleleDepthUtil: depthIntToByte: Can not have negative depth values: -8448

在建树之前这步对过滤后vcf文件排序时出现这个报错,哪位大佬知道啥吗

文章 biolinux安装matplotlib时报错

biolinuxUbuntu系统上安装matplotlib时报错: sudo pip install matplotlib 遇到报错: Downloading tornado-5.0.2.tar.gz (506kB): 506kB downloaded   Running setup.py (path:/tmp/pip_build_root/tornado/setup.py) egg_info for package tornado     Traceback (most recent call last): ...

问题 我在文献中读到这么一段话,是关于某一基因家族在某植物各个组织器官中表达,请老师帮我看看这是怎么做

...ues were log2-transformed. 这是描述做植物各个组织器官各基因表达量,请问这是怎么做

问题 Linux中FOR循环做BWA比对报错[main_samview] fail to read the header from "-".

按照重测序课程中命令 for i in CB2 CB3; do echo  "RUN CMD: bwa mem  Prunus_dulcis.T2T.assembly.fa ${i}_1.fq.gz \ ${i}_2.fq.gz -t 10 -M \ -R '@RG\tID:${i}\tLB:${i}\tPL:ILLUMINA\tSM:${i}' \ |samtools view -bS -h - > ${i}.bam" nohup bwa mem  Prunus_dulcis.T2T.assembly.fa ${i}_...

问题 Error correction and assembling warnings: * 0:12:28.935 6768M / 13G WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 16

请问您这个谷值问题怎么解决

文章 docker启动报错:Error starting daemon: SELinux is not supported with the overlay2 graph driver on this ke

...ker (--selinux-enabled=false) 解决方案: 意思是说:此linux内核中SELinux不支持 overlay2 graph driver ,解决方法有两个,要么启动一个新内核,要么就在docker里禁用selinux,--selinux-enabled=false 重新编辑docker配置文件: vi /etc/sysco...

文章 R 安装包:configure: error: proj_api.h not found in standard or given locations.

...’* removing ‘/share/work/biosoft/R/R-v4.3.1/lib64/R/library/sf’下载程序包在‘/tmp/Rtmptf5D4o/downloaded_packages’里 解决办法: yum install  proj   proj-devel