找到约 15 条结果

文章 seqkit序列处理神器的常用命令

...a/q file manipulation. PloS One 11, e0163962, doi: https://doi.org/10.1371/journal.pone.0163962 软件发布页:https://github.com/shenwei356/seqkit/releases 帮助文档:https://bioinf.shenwei.me/seqkit/ 参考:https://www.cnblogs.com/huangyinger/p/10421805.html

问题 Error running system command:

...gt; NOTICE: Finished reading 5099177 lines from VCF file NOTICE: A total of 5099109 locus in VCF file passed QC threshold, representing 5099109 SNPs (3715827 transitions and 1383282 transversions) and 0 indels/substitutions NOTICE: Finished writing allele frequencies based on 2365986576 SNP genot...

问题 用GATK call SNP时报错

...ease index all input files: samtools index SRR3274663.new.sorted.bam Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace. 这是我的命令:gatk --java-options "-Xmx20g -Djava.io.tmpdir=./tmp" HaplotypeC...

问题 老师,请问CGVIEW中的gene.blast文件格式

...,望你解答。谢谢! 报错和blast文件如下: Cannot parse the #PROGRAM field in the BLAST results file ./YC_bacteria_cgview/blast.txt at ./scripts/cgview_xml_builder/cgview_xml_builder.pl line 4716, <INFILE> line 0.000000. #PROGRAMquery_id    match_id    match_descripti...

文章 bam文件转换fastq

...eating paired FASTQ.        -tags   Create FASTQ based on the mate info                in the BAM R2 and Q2 tags.Tips:        If you want to create a single, interleaved FASTQ file        for paired-end data, you can just write...

问题 WGCNA-MAD

...################################## input data########################## # The following setting is important, do not omit. options(stringsAsFactors = FALSE) #读取数据,删除第一列GeneSymbol datExpr = read.table(expr_file,header=T,row.names=1,comment.char = "",check.names=F) datExpr = d...

文章 轻松下载k8s.gcr.io,gcr.io,quay.io镜像

...下所示:[root@k8scloude2 ~]# docker pull quay.io/kubevirt/virt-api:v0.45.0 #换成 [root@k8scloude2 ~]# docker pull quay.mirrors.ustc.edu.cn/kubevirt/virt-api:v0.45.0

文章 转录组后续实验怎么做?

...二篇SCI,欢迎您持续关注! 参考文献:Characterization of the Asiatic Acid Glucosyltransferase, UGT73AH1, Involved in Asiaticoside Biosynthesis in Centella asiatica(L.) Urban. Int. J. Mol. Sci. 2017, 18, 2630    日常科研中你我经会常遇到看不懂的图表,不会挖...

文章 MENA如何结合OTU共现性网络与环境因子

...生物OTU网络图绘制(Cytoscape) MENA在完成构建网络(Construct the network)之后,可以对网络进行分析(Analyze the networks),其中一项是结合性状数据,又或者说环境性状(因子)进行联合分析。 其主要步骤如下: 1、 点击网页左侧A...

问题 重复序列分析,去除含有基因序列的库文件时候报错,你们示例数据也有这样的问题

...$(grep -c ">" ${lib}noProtFinal)"done 会报错如下: Can not open the seqfile ModelerAll.lib_blast_results.txt.fnolowm50seqmergeunmatchedregion.pl seqfileIllegal division by zero at /public-supool/home/thli/scripts/genome_annotation/ProtExcluder1.2/GCcontent.pl line 122.Can not open the seq...

文章 mclust analysis.r 基于模型的聚类

... input clinical information file path[required] --mclust whether to cluster the samples[optional,default:False] -g group, --group group Group the samples into several categories[optional,default:3] -n model_name, --model_name model_n...

文章 topGO报错

...s) <- names(termsNames)                    ## return NAs for the terms that are not found in the DB and make sure the 'names' attribute is as specified          shortNames <- shortNames[whichTerms]          names(shortNames) <- whichTerms                    r...

问题 无法链接到docker守护进程

docker 容器自动退出,再连就报错:Cannot connect to the Docker daemon at unix:///var/run/docker.sock. Is the docker daemon running? 我检查docker运行状态显示正在运行: docker.service - Docker Application Container Engine Loaded: loaded (/lib/systemd/system/docker.servic...

问题 无法链接到docker守护进程

docker 容器自动退出,再连就报错:Cannot connect to the Docker daemon at unix:///var/run/docker.sock. Is the docker daemon running? 我检查docker运行状态显示正在运行: docker.service - Docker Application Container Engine Loaded: loaded (/lib/systemd/system/docker.servic...

问题 用string网站预测蛋白质互作网络,为什么相隔两个月预测的两次结果不一致?

...白质互作网络,六月底进行了一次,hide disconnected nodes in the network后有40个节点,隐藏了20个节点;八月低在string进行了同样的操作,hide disconnected nodes in the network后有63个节点,节点数没有减少;这两次使用的是同一个蛋白序列fa...