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问题 共线性分析画圈图的时候出现报错Width (0) and height (0) cannot be

Reading control file error: For input string: "1000    //plot width and height (in pixels)"Exception in thread "main" java.lang.IllegalArgumentException: Width (0) and height (0) cannot be <= 0    at java.desktop/java.awt.image.DirectColorModel.createCompatibleWritableRaster(DirectColorMode...

文章 MA plot 绘图计算公式

...MA图绘制: affy (ma.plot, mva.pairs), limma (plotMA), marray (maPlot), and edgeR(maPlot) 原文参考维基百科:MA plot (点击进入页面) ############## Microarray data is often normalized within arrays to control for systematic biases in dye coupling and hybridization efficiencies...

文章 SGE添加计算节点步骤

... Engine using the sgepasswd client application. (See Appendix B for more information.) Log in to the execution host as root. As you did for the master installation, either copy the installation files to a local installation directory sge-root or use a network installation directory. ...

文章 network.r绘制网络图并输出最大子图节点及网络图

...exit -e filepath, --edge filepath input the edge information of network,[required] -v filepath, --vertex filepath input the vertex information of network,[required] -f from, --from from the name of the start gene column [required] -t to, --to ...

文章 bedtools批量提取基因组指定位置序列

... will be reverse complemented.                - By default, strand information is ignored.        -fullHeader     Use full fasta header.                - By default, only the word before the first space or tab is used. 其中-fi 指定基因组fasta文件,-bed 指定要提...

文章 癌症缺氧相关基因查找,以及样本分类方法 ,免疫微环境

...SigDB version 6.0). MCP-counter scores regarding immune-related activity and fibroblasts were evaluated using the ‘MCPcounter’ package in R [40]. ssGSEA was performed to calculate the enrichment score of specific immune signatures in samples using the ‘GSVA’ package in R according to a...

文章 NCBI 蛋白数据库分库

...             |4       |       PLN     |       Plants and Fungi        |               |5       |       PRI     |       Primates        |               |6       |       ROD     |       Rodents |               |7    ...

问题 我买了转录组分析的课程,在运行fpkm_and_plot.R脚本时提示Error in library("getopt") : there is no package called ‘getopt’ Execution halted, 我打开R语言安装getopt后仍然显示报错,请教各位大神和组学讲堂的老师

文章 z-Transform数据得到z-score方法公式

...le observationsxi ... original values of the sample... sample means ... standard deviation of the sample The z-transform is also called standardization or auto-scaling. z-Scores become comparable by measuring the observations in multiples of the standard deviation of that sample. The mean o...

问题 成功安装WGCNA包后,调用时报错

... bytes (3.3 MB) downloaded 3.3 MBpackage ‘WGCNA’ successfully unpacked and MD5 sums checkedThe downloaded binary packages are in C:\Users\NY\AppData\Local\Temp\RtmpYrZpG5\downloaded_packages installing the source package ‘GO.db’trying URL 'https://bioconductor.org/packages/3.8/data/annotati...

文章 rocks7 SGE任务管理配置

...mputing clusters today. Users should use use qmon (configure SGE by GUI) and qconf (configure SEG by command line) to configure SGE.Detailed ReferenceThe more authorative and comprehensive reference is http://gridscheduler.sourceforge.net/htmlman/htmlman1/qstat.html and http://gridscheduler.s...

文章 immune_infiltrates_rnaseq.r RNA-seq 转录组数据免疫侵润分析

... “In a certain patient, there are more B cells than T cells”. For more information, see our Benchmark paper (Sturm et al. (2019)).Methods that allow between-sample comparisonsMCP-counter xCell TIMERMethods that allow between-cell-type comparisionsCIBERSORTMethods that allow bothEPIC quanTIseq ...

文章 stacks RAD 分析方法

...carding reads of low quality (-q), removing reads with uncalled bases (-c) and rescuing barcodes and radtags (-r). SNP calling for each individual was done using the program denovo_map.pl of Stacks. The denovo_map.pl included six core components: building loci (ustacks), creating a catalog of lo...

文章 影响GWAS关联分析好坏的因素

...cs, 2012, 44(7): 803-807 4.Yu J, Buckler E S. Genetic association mapping and genome organization of maize[J]. Current Opinion in Biotechnology, 2006, 17(2):155–160. 5.Mcmullen M D, Stephen K, Hector Sanchez V, et al. Genetic Properties of the Maize Nested Association Mapping Population[J]. Scie...

问题 您这里的课程筛选串联重复基因的标准是参照这篇文献的extent of gene duplication in the genomes of Drosophila,nematode ,and yeast(2002). Mol Biol Evol。 这篇文献是用的果蝇,线虫,和酵母菌的,可我做的是植物,我还可以用这种方法吗